AUTHOR=Mu Yanjun , Gong Wenping , Qie Yanmin , Liu Xueqing , Li Linzhi , Sun Nina , Liu Wei , Guo Jun , Han Ran , Yu Ziyang , Xiao Luning , Su Fuyu , Zhang Wenjing , Wang Jiangchun , Han Guohao , Ma Pengtao 

TITLE=Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1005627

DOI=10.3389/fpls.2022.1005627

ISSN=1664-462X

ABSTRACT=<p>Powdery mildew of wheat (<italic>Triticum aestivum</italic>), caused by <italic>Blumeria graminis</italic> f.sp. <italic>tritici</italic> (<italic>Bgt</italic>), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using <italic>Bgt</italic> isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated <italic>pmYN99102</italic>, conferred seedling resistance to the <italic>Bgt</italic> isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), <italic>pmYN99102</italic> was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, <italic>pmYN99102</italic> differed from those of the reported powdery mildew (<italic>Pm</italic>) resistance genes on 2BL, suggesting <italic>pmYN99102</italic> is most likely a new <italic>Pm</italic> gene/allele in the targeted interval. To transfer <italic>pmYN99102</italic> to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for <italic>YTU103-97</italic> can be used in MAS for tracking <italic>pmYN99102</italic> when it transferred into those susceptible cultivars.</p>