AUTHOR=Guo Xiaoyang , Jiang Jinghan , Liu Ying , Yu Lili , Chang Ruzhen , Guan Rongxia , Qiu Lijuan 

TITLE=Identification of a Novel Salt Tolerance-Related Locus in Wild Soybean (Glycine soja Sieb. & Zucc.)

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.791175

DOI=10.3389/fpls.2021.791175

ISSN=1664-462X

ABSTRACT=<p>Salinity is an important abiotic stress factor that affects growth and yield of soybean. NY36-87 is a wild soybean germplasm with high salt tolerance. In this study, two F<sub>2:3</sub> mapping populations derived from NY36-87 and two salt-sensitive soybean cultivars, Zhonghuang39 and Peking, were used to map salt tolerance-related genes. The two populations segregated as 1 (tolerant):2 (heterozygous):1 (sensitive), indicating a Mendelian segregation model. Using simple sequence repeat (SSR) markers together with the bulked segregant analysis (BSA) mapping strategy, we mapped a salt tolerance locus on chromosome 03 in F<sub>2:3</sub> population Zhonghuang39×NY36-87 to a 98-kb interval, in which the known gene <italic>GmSALT3</italic> co-segregated with the salt tolerance locus. In the F<sub>2:3</sub> population of Peking×NY36-87, the dominant salt tolerance-associated gene was detected and mapped on chromosome 18. We named this gene <italic>GmSALT18</italic> and fine mapped it to a 241-kb region. Time course analysis and a grafting experiment confirmed that Peking accumulated more Na<sup>+</sup> in the shoot <italic>via</italic> a root-based mechanism. These findings reveal that the tolerant wild soybean line NY36-87 contains salt tolerance-related genes <italic>GmSALT3</italic> and <italic>GmSALT18</italic>, providing genetic material and a novel locus for breeding salt-tolerant soybean.</p>