AUTHOR=Schrauf Gustavo E. , Voda Lisandro , Zelada Alicia M. , García Ana María , Giordano Andrea , Roa Pablo Peralta , Guitian Juan , Rebori Juan , Ghio Sergio , Couso Luciana , Castro Lautaro , Musacchio Eduardo , Rush Pablo , Nagel Jutta , Wang Zeng Yu , Cogan Noel , Spangenberg Germán 

TITLE=Development of Protocols for Regeneration and Transformation of Apomitic and Sexual Forms of Dallisgrass (Paspalum dilatatum Poir.)

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.787549

DOI=10.3389/fpls.2021.787549

ISSN=1664-462X

ABSTRACT=<p><italic>Paspalum dilatatum</italic> (common name dallisgrass), a productive C4 grass native to South America, is an important pasture grass found throughout the temperate warm regions of the world. It is characterized by its tolerance to frost and water stress and a higher forage quality than other C4 forage grasses. <italic>P. dilatatum</italic> includes tetraploid (2<italic>n</italic> = 40), sexual, and pentaploid (2<italic>n</italic> = 50) apomictic forms, but is predominantly cultivated in an apomictic monoculture, which implies a high risk that biotic and abiotic stresses could seriously affect the grass productivity. The obtention of reproducible and efficient protocols of regeneration and transformation are valuable tools to obtain genetic modified grasses with improved agronomics traits. In this review, we present the current regeneration and transformation methods of both apomictic and sexual cultivars of <italic>P. dilatatum</italic>, discuss their strengths and limitations, and focus on the perspectives of genetic modification for producing new generation of forages. The advances in this area of research lead us to consider <italic>Paspalum dilatatum</italic> as a model species for the molecular improvement of C4 perennial forage species.</p>