AUTHOR=Siddiqui Zamin Shaheed , Lee Gang-Seob , Cho Woosuk , Jeong Mi-Jeong , Park Soo-Chul , Kwon Taek-Ryoun , Zulfiqar Faisal , Umar Muhammad , Abideen Zainul , Uddin Zaheer , Ansari Hafiza Hamna , Wajid Danish , Cho Jung-Il 

TITLE=Physiological Aspects of Germination and Early Seedling Establishment of Pleurotus sajor-caju Glyceraldehyde-3-Phosphate Dehydrogenase Expressing Transgenic Rice in Saline Environment

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.767826

DOI=10.3389/fpls.2021.767826

ISSN=1664-462X

ABSTRACT=<p><italic>GPD</italic> encodes glyceraldehyde-3-phosphate dehydrogenase enzyme involved in sugar mobilization, particularly glycolysis and gluconeogenesis. The objective of this study was to determine physiological aspects of germination and early seedling establishment of <italic>PsGPD</italic> (<italic>Pleurotus sajor-caju</italic> glyceraldehyde-3-phosphate dehydrogenase) expressing transgenic rice (T5) against different salt concentrations. The T5 line that carried 2 copies of T-DNA and had the highest level of <italic>PsGPD</italic> expression was used in the investigation. Final germination percentage, amylase activity, reducing sugar accumulation, and chlorophyll biosynthesis were comparatively higher in <italic>PsGPD</italic> expressing transgenic rice against elevating saline conditions. A slow-paced conversion of porphyrin's precursors was seen through the matrix model and further elaborated by a graphical model. A sustained level of porphyrin was observed in <italic>PsGPD</italic> expressing transgenic rice. These data were concurrent with the relative gene expression and thermal imaging (thermography) of <italic>PsGPD</italic> expressing transgenic rice against salt stress. Morphological attributes also favored the salt tolerance exhibited by <italic>PsGPD-</italic>transformed rice.</p>