AUTHOR=Golisz Anna , Krzyszton Michal , Stepien Monika , Dolata Jakub , Piotrowska Justyna , Szweykowska-Kulinska Zofia , Jarmolowski Artur , Kufel Joanna 

TITLE=Arabidopsis Spliceosome Factor SmD3 Modulates Immunity to Pseudomonas syringae Infection

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.765003

DOI=10.3389/fpls.2021.765003

ISSN=1664-462X

ABSTRACT=<p>SmD3 is a core component of the small nuclear ribonucleoprotein (snRNP) that is essential for pre-mRNA splicing. The role of <italic>Arabidopsis</italic> SmD3 in plant immunity was assessed by testing sensitivity of <italic>smd3a</italic> and <italic>smd3b</italic> mutants to <italic>Pseudomonas syringae</italic> pv. <italic>tomato</italic> (<italic>Pst</italic>) DC3000 infection and its pathogenesis effectors flagellin (flg22), EF-Tu (elf18) and coronatine (COR). Both <italic>smd3</italic> mutants exhibited enhanced susceptibility to <italic>Pst</italic> accompanied by marked changes in the expression of key pathogenesis markers. mRNA levels of major biotic stress response factors were also altered upon treatment with <italic>Pseudomonas</italic> effectors. Our genome-wide transcriptome analysis of the <italic>smd3b-1</italic> mutant infected with <italic>Pst</italic>, verified by northern and RT-qPCR, showed that lack of SmD3-b protein deregulates defense against <italic>Pst</italic> infection at the transcriptional and posttranscriptional levels including defects in splicing and an altered pattern of alternative splicing. Importantly, we show that SmD3-b dysfunction impairs mainly stomatal immunity as a result of defects in stomatal development. We propose that it is the malfunction of the stomata that is the primary cause of an altered mutant response to the pathogen. Other changes in the <italic>smd3b-1</italic> mutant involved enhanced elf18- and flg22-induced callose deposition, reduction of flg22-triggered production of early ROS and boost of secondary ROS caused by <italic>Pst</italic> infection. Together, our data indicate that SmD3 contributes to the plant immune response possibly <italic>via</italic> regulation of mRNA splicing of key pathogenesis factors.</p>