AUTHOR=Wang Panpan , Yang Lifang , Sun Jialing , Yang Ye , Qu Yuan , Wang Chengxiao , Liu Diqiu , Huang Luqi , Cui Xiuming , Liu Yuan 

TITLE=Structure and Function of Rhizosphere Soil and Root Endophytic Microbial Communities Associated With Root Rot of Panax notoginseng

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.752683

DOI=10.3389/fpls.2021.752683

ISSN=1664-462X

ABSTRACT=<p><italic>Panax notoginseng</italic> (Burk.) F. H. Chen is a Chinese medicinal plant of the Araliaceae family used for the treatment of cardiovascular and cerebrovascular diseases in Asia. <italic>P. notoginseng</italic> is vulnerable to root rot disease, which reduces the yield of <italic>P. notoginseng</italic>. In this study, we analyzed the rhizosphere soil and root endophyte microbial communities of <italic>P. notoginseng</italic> from different geographical locations using high-throughput sequencing. Our results revealed that the <italic>P. notoginseng</italic> rhizosphere soil microbial community was more diverse than the root endophyte community. <italic>Rhodopseudomonas</italic>, <italic>Actinoplanes</italic>, <italic>Burkholderia</italic>, and <italic>Variovorax paradoxus</italic> can help <italic>P. notoginseng</italic> resist the invasion of root rot disease. <italic>Ilyonectria mors-panacis</italic>, <italic>Pseudomonas fluorescens</italic>, and <italic>Pseudopyrenochaeta lycopersici</italic> are pathogenic bacteria of <italic>P. notoginseng</italic>. The upregulation of amino acid transport and metabolism in the soil would help to resist pathogens and improve the resistance of <italic>P. notoginseng.</italic> The ABC transporter and gene modulating resistance genes can improve the disease resistance of <italic>P. notoginseng</italic>, and the increase in the number of GTs (glycosyltransferases) and GHs (glycoside hydrolases) families may be a molecular manifestation of <italic>P. notoginseng</italic> root rot. In addition, the complete genomes of two <italic>Flavobacteriaceae</italic> species and one <italic>Bacteroides</italic> species were obtained. This study demonstrated the microbial and functional diversity in the rhizosphere and root microbial community of <italic>P. notoginseng</italic> and provided useful information for a better understanding of the microbial community in <italic>P. notoginseng</italic> root rot. Our results provide insights into the molecular mechanism underlying <italic>P. notoginseng</italic> root rot and other plant rhizosphere microbial communities.</p>