AUTHOR=Li Song , Liu Zihui , Guo Linlin , Li Hongjie , Nie Xiaojun , Chai Shoucheng , Zheng Weijun 

TITLE=Genome-Wide Identification of Wheat ZIP Gene Family and Functional Characterization of the TaZIP13-B in Plants

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.748146

DOI=10.3389/fpls.2021.748146

ISSN=1664-462X

ABSTRACT=<p>The ZIP (Zn-regulated, iron-regulated transporter-like protein) transporter plays an important role in regulating the uptake, transport, and accumulation of microelements in plants. Although some studies have identified ZIP genes in wheat, the significance of this family is not well understood, particularly its involvement under Fe and Zn stresses. In this study, we comprehensively characterized the wheat ZIP family at the genomic level and performed functional verification of three TaZIP genes by yeast complementary analysis and of <italic>TaZIP13-B</italic> by transgenic <italic>Arabidopsis</italic>. Totally, 58 TaZIP genes were identified based on the genome-wide search against the latest wheat reference (IWGSC_V1.1). They were then classified into three groups, based on phylogenetic analysis, and the members within the same group shared the similar exon-intron structures and conserved motif compositions. Expression pattern analysis revealed that the most of TaZIP genes were highly expressed in the roots, and nine TaZIP genes displayed high expression at grain filling stage. When exposed to ZnSO<sub>4</sub> and FeCl<sub>3</sub> solutions, the TaZIP genes showed differential expression patterns. Additionally, six ZIP genes responded to zinc-iron deficiency. A total of 57 miRNA-TaZIP interactions were constructed based on the target relationship, and three miRNAs were downregulated when exposed to the ZnSO<sub>4</sub> and FeCl<sub>3</sub> stresses. Yeast complementation analysis proved that <italic>TaZIP14-B</italic>, <italic>TaZIP13-B</italic>, and <italic>TaIRT2-A</italic> could transport Zn and Fe. Finally, overexpression of <italic>TaZIP13-B</italic> in <italic>Arabidopsis</italic> showed that the transgenic plants displayed better tolerance to Fe/Zn stresses and could enrich more metallic elements in their seeds than wild-type <italic>Arabidopsis</italic>. This study systematically analyzed the genomic organization, gene structure, expression profiles, regulatory network, and the biological function of the ZIP family in wheat, providing better understanding of the regulatory roles of TaZIPs and contributing to improve nutrient quality in wheat crops.</p>