AUTHOR=Wang Xiaohui , Li Junfeng , Li Fei , Pan Yu , Cai Dan , Mao Dandan , Chen Liangbi , Luan Sheng TITLE=Rice Potassium Transporter OsHAK8 Mediates K+ Uptake and Translocation in Response to Low K+ Stress JOURNAL=Frontiers in Plant Science VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.730002 DOI=10.3389/fpls.2021.730002 ISSN=1664-462X ABSTRACT=

Potassium (K⁺) levels in the soil often limit plant growth and development. As a result, crop production largely relies on the heavy use of chemical fertilizers, presenting a challenging problem in sustainable agriculture. To breed crops with higher K⁺-use efficiency (KUE), we must learn how K⁺ is acquired from the soil by the root system and transported to the rest of the plant through K⁺ transporters. In this study, we identified the function of the rice K⁺ transporter OsHAK8, whose expression level is downregulated in response to low-K⁺ stress. When OsHAK8 was disrupted by CRISPR/Cas9-mediated mutagenesis, Oshak8 mutant plants showed stunted growth, especially under low-K⁺ conditions. Ion content analyses indicated that K⁺ uptake and root-to-shoot K⁺ transport were significantly impaired in Oshak8 mutants under low-K⁺ conditions. As the OsHAK8 gene was broadly expressed in different cell types in the roots and its protein was targeted to the plasma membrane, we propose that OsHAK8 serves as a major transporter for both uptake and root-to-shoot translocation in rice plants.