AUTHOR=Wang Xiaolu , Yu Zhihui , Wang Hongjin , Li Jianbo , Han Ran , Xu Wenjing , Li Guangrong , Guo Jun , Zi Yan , Li Faji , Cheng Dungong , Liu Aifeng , Li Haosheng , Yang Zujun , Liu Jianjun , Liu Cheng 

TITLE=Characterization, Identification and Evaluation of Wheat-Aegilops sharonensis Chromosome Derivatives

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.708551

DOI=10.3389/fpls.2021.708551

ISSN=1664-462X

ABSTRACT=<p><italic>Aegilops sharonensis</italic>, a wild relative of wheat, harbors diverse disease and insect resistance genes, making it a potentially excellent gene source for wheat improvement. In this study, we characterized and evaluated six wheat-<italic>A. sharonensis</italic> derivatives, which included three disomic additions, one disomic substitution + monotelosomic addition and two disomic substitution + disomic additions. A total of 51 PLUG markers were developed and used to allocate the <italic>A. sharonensis</italic> chromosomes in each of the six derivatives to <italic>Triticeae</italic> homoeologous groups. A set of cytogenetic markers specific for <italic>A. sharonensis</italic> chromosomes was established based on FISH using oligonucleotides as probes. Molecular cytogenetic marker analysis confirmed that these lines were a CS-<italic>A</italic>. <italic>sharonensis</italic> 2S<sup>sh</sup> disomic addition, a 4S<sup>sh</sup> disomic addition, a 4S<sup>sh</sup> (4D) substitution + 5S<sup>sh</sup>L monotelosomic addition, a 6S<sup>sh</sup> disomic addition, a 4S<sup>sh</sup> (4D) substitution + 6S<sup>sh</sup> disomic addition and a 4S<sup>sh</sup> (4D) substitution + 7S<sup>sh</sup> disomic addition line, respectively. Disease resistance investigations showed that chromosome 7S<sup>sh</sup> of <italic>A</italic>. <italic>sharonensis</italic> might harbor a new powdery mildew resistance gene, and therefore it has potential for use as resistance source for wheat breeding.</p>