AUTHOR=Ce Fuquan , Mei Jiaqin , He Haiyan , Zhao Yu , Hu Wenhui , Yu Fengqun , Li Qinfei , Ren Xuesong , Si Jun , Song Hongyuan , Qian Wei 

TITLE=Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.703520

DOI=10.3389/fpls.2021.703520

ISSN=1664-462X

ABSTRACT=<p>Clubroot caused by <italic>Plasmodiophora brassicae</italic> is a devastating disease of cabbage (<italic>Brassica oleracea</italic>). To identify quantitative trait loci (QTLs) for clubroot resistance (CR) in <italic>B. oleracea</italic>, genomic resequencing was carried out in two sets of extreme pools, group I and group II, which were constructed separately from 110 and 74 F2 cloned lines derived from the cross between clubroot-resistant (R) cabbage “GZ87” (against race 4) and susceptible (S) cabbage “263.” Based on the QTL-<italic>sequencing</italic> (QTL-Seq) analysis of group I and group II, three QTLs (i.e., <italic>qCRc7-2, qCRc7-3</italic>, and <italic>qCRc7-4</italic>) were determined on the C07 chromosome. RNA-Seq and qRT-PCR were conducted in the extreme pools of group II before and after inoculation, and two potential candidate genes (i.e., <italic>Bol037115</italic> and <italic>Bol042270</italic>), which exhibiting upregulation after inoculation in the R pool but downregulation in the S pool, were identified from the three QTLs on C07. A functional marker “SWU-OA” was developed from <italic>qCRc7-4</italic> on C07, exhibiting ∼95% accuracy in identifying CR in 56 F2 lines. Our study will provide valuable information on resistance genes against <italic>P. brassicae</italic> and may accelerate the breeding process of <italic>B. oleracea</italic> with CR.</p>