AUTHOR=Kong Mengmeng , Wu Yaozong , Wang Ziyuan , Qu Wantong , Lan Yixin , Chen Xin , Liu Yanyun , Shahnaz Perveen , Yang Zhongnan , Yu Qingbo , Mi Hualing 

TITLE=A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development in Arabidopsis thaliana

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.700975

DOI=10.3389/fpls.2021.700975

ISSN=1664-462X

ABSTRACT=<p>Chloroplast development involves the coordinated expression of both plastids- and nuclear-encoded genes in higher plants. However, the underlying mechanism still remains largely unknown. In this study, we isolated and characterized an <italic>Arabidopsis</italic> mutant with an albino lethality phenotype named <italic>RNA processing 8</italic> (<italic>rp8</italic>). Genetic complementation analysis demonstrated that the gene <italic>AT4G37920</italic> (<italic>RP8</italic>) was responsible for the mutated phenotype. The <italic>RP8</italic> gene was strongly expressed in photosynthetic tissues at both transcription and translation protein levels. The RP8 protein is localized in the chloroplast and associated with the thylakoid. Disruption of the <italic>RP8</italic> gene led to a defect in the accumulation of the <italic>rpoA</italic> mature transcript, which reduced the level of the RpoA protein, and affected the transcription of PEP-dependent genes. The abundance of the chloroplast rRNA, including <italic>23S</italic>, <italic>16S</italic>, <italic>4.5S</italic>, and <italic>5S rRNA</italic>, were reduced in the <italic>rp8</italic> mutant, respectively, and the amounts of chloroplast ribosome proteins, such as, PRPS1(uS1c), PRPS5(uS5c), PRPL2 (uL2c), and PRPL4 (uL4c), were substantially decreased in the <italic>rp8</italic> mutant, which indicated that knockout of RP8 seriously affected chloroplast translational machinery. Accordingly, the accumulation of photosynthetic proteins was seriously reduced. Taken together, these results indicate that the RP8 protein plays an important regulatory role in the <italic>rpoA</italic> transcript processing, which is required for the expression of chloroplast genes and chloroplast development in <italic>Arabidopsis</italic>.</p>