AUTHOR=Li Zhong-Qing , Zhang Yao , Li He , Su Ting-Ting , Liu Cheng-Gong , Han Zi-Chao , Wang Ai-Ying , Zhu Jian-Bo 

TITLE=Genome-Wide Characterization and Expression Analysis Provide Basis to the Biological Function of Cotton FBA Genes

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.696698

DOI=10.3389/fpls.2021.696698

ISSN=1664-462X

ABSTRACT=<p>Fructose-1,6-biphosphate aldolase (FBA) is a multifunctional enzyme in plants, which participates in the process of Calvin-Benson cycle, glycolysis and gluconeogenesis. Despite the importance of <italic>FBA</italic> genes in regulating plant growth, development and abiotic stress responses, little is known about their roles in cotton. In the present study, we performed a genome-wide identification and characterization of <italic>FBAs</italic> in <italic>Gossypium hirsutum</italic>. Totally seventeen <italic>GhFBA</italic> genes were identified. According to the analysis of functional domain, phylogenetic relationship, and gene structure, <italic>GhFBA</italic> genes were classified into two subgroups. Furthermore, nine <italic>GhFBAs</italic> were predicted to be in chloroplast and eight were located in cytoplasm. Moreover, the promoter prediction showed a variety of abiotic stresses and phytohormone related <italic>cis</italic>-acting elements exist in the 2k up-stream region of <italic>GhFBA</italic>. And the evolutionary characteristics of cotton <italic>FBA</italic> genes were clearly presented by synteny analysis. Moreover, the results of transcriptome and qRT-PCR analysis showed that the expression of <italic>GhFBAs</italic> were related to the tissue distribution, and further analysis suggested that <italic>GhFBAs</italic> could respond to various abiotic stress and phytohormonal treatments. Overall, our systematic analysis of <italic>GhFBA</italic> genes would not only provide a basis for the understanding of the evolution of <italic>GhFBAs</italic>, but also found a foundation for the further function analysis of <italic>GhFBAs</italic> to improve cotton yield and environmental adaptability.</p>