AUTHOR=Dong Xiaonan , Liu Chaorui , Wang Yuqi , Dong Qing , Gai Yingping , Ji Xianling 

TITLE=MicroRNA Profiling During Mulberry (Morus atropurpurea Roxb) Fruit Development and Regulatory Pathway of miR477 for Anthocyanin Accumulation

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.687364

DOI=10.3389/fpls.2021.687364

ISSN=1664-462X

ABSTRACT=<p>To understand the mechanism of small non-coding RNAs (miRNA)-mediated development and ripening of mulberry fruits, three small RNA libraries from mulberry fruits at different development stages were constructed, and 159 conserved miRNAs as well as 86 novel miRNAs were successfully identified. Among the miRNAs identified, there were 90 miRNAs which showed differential expression patterns at different stages of fruit development and ripening. The target genes of these differential expressed (DE) miRNAs were involved in growth and development, transcription and regulation of transcription, metabolic processes, and etc. Interestingly, it was found that the expression level of mul-miR477 was increased with fruit ripening, and it can target the antisense lncRNA (<italic>Mul-ABCB19AS</italic>) of the ATP binding cassette (ABC) transporter B 19 gene (<italic>Mul-ABCB19</italic>). Our results showed that mul-miR477 can repress the expression of <italic>Mul-ABCB19AS</italic> and increase the expression of <italic>Mul-ABCB19</italic>, and it acted as a positive regulator participating anthocyanin accumulation through the regulatory network of mul-miR477—<italic>Mul-ABCB19AS</italic>—<italic>Mul-ABCB19</italic>.</p>