AUTHOR=Aono Alexandre Hild , Pimenta Ricardo José Gonzaga , Garcia Ana Letycia Basso , Correr Fernando Henrique , Hosaka Guilherme Kenichi , Carrasco Marishani Marin , Cardoso-Silva Cláudio Benício , Mancini Melina Cristina , Sforça Danilo Augusto , dos Santos Lucas Borges , Nagai James Shiniti , Pinto Luciana Rossini , Landell Marcos Guimarães de Andrade , Carneiro Monalisa Sampaio , Balsalobre Thiago Willian , Quiles Marcos Gonçalves , Pereira Welison Andrade , Margarido Gabriel Rodrigues Alves , de Souza Anete Pereira TITLE=The Wild Sugarcane and Sorghum Kinomes: Insights Into Expansion, Diversification, and Expression Patterns JOURNAL=Frontiers in Plant Science VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.668623 DOI=10.3389/fpls.2021.668623 ISSN=1664-462X ABSTRACT=

The protein kinase (PK) superfamily is one of the largest superfamilies in plants and the core regulator of cellular signaling. Despite this substantial importance, the kinomes of sugarcane and sorghum have not been profiled. Here, we identified and profiled the complete kinomes of the polyploid Saccharum spontaneum (Ssp) and Sorghum bicolor (Sbi), a close diploid relative. The Sbi kinome was composed of 1,210 PKs; for Ssp, we identified 2,919 PKs when disregarding duplications and allelic copies, and these were related to 1,345 representative gene models. The Ssp and Sbi PKs were grouped into 20 groups and 120 subfamilies and exhibited high compositional similarities and evolutionary divergences. By utilizing the collinearity between the species, this study offers insights into Sbi and Ssp speciation, PK differentiation and selection. We assessed the PK subfamily expression profiles via RNA-Seq and identified significant similarities between Sbi and Ssp. Moreover, coexpression networks allowed inference of a core structure of kinase interactions with specific key elements. This study provides the first categorization of the allelic specificity of a kinome and offers a wide reservoir of molecular and genetic information, thereby enhancing the understanding of Sbi and Ssp PK evolutionary history.