AUTHOR=Fedorchuk Tatiana P. , Kireeva Inga A. , Opanasenko Vera K. , Terentyev Vasily V. , Rudenko Natalia N. , Borisova-Mubarakshina Maria M. , Ivanov Boris N. 

TITLE=Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in Isolated Arabidopsis Thylakoids

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.662082

DOI=10.3389/fpls.2021.662082

ISSN=1664-462X

ABSTRACT=<p>We studied bicarbonate-induced stimulation of photophosphorylation in thylakoids isolated from leaves of <italic>Arabidopsis thaliana</italic> plants. This stimulation was not observed in thylakoids of wild-type in the presence of mafenide, a soluble carbonic anhydrase inhibitor, and was absent in thylakoids of two mutant lines lacking the gene encoding alpha carbonic anhydrase 5 (αCA5). Using mass spectrometry, we revealed the presence of αCA5 in stromal thylakoid membranes of wild-type plants. A possible mechanism of the photophosphorylation stimulation by bicarbonate that involves αCA5 is proposed.</p>