AUTHOR=Wang Yingfeng , Dai Xiaoyan , Xu Gaoqiang , Dai Zhuoyi , Chen Peiyu , Zhang Tongjin , Zhang Huifen TITLE=The Ca2+-CaM Signaling Pathway Mediates Potassium Uptake by Regulating Reactive Oxygen Species Homeostasis in Tobacco Roots Under Low-K+ Stress JOURNAL=Frontiers in Plant Science VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.658609 DOI=10.3389/fpls.2021.658609 ISSN=1664-462X ABSTRACT=

Potassium (K⁺) deficiency severely threatens crop growth and productivity. Calcium (Ca²⁺) signaling and its sensors play a central role in the response to low-K⁺ stress. Calmodulin (CaM) is an important Ca²⁺ sensor. However, the mechanism by which Ca²⁺ signaling and CaM mediate the response of roots to low-K⁺ stress remains unclear. In this study, we found that the K⁺ concentration significantly decreased in both shoots and roots treated with Ca²⁺ channel blockers, a Ca²⁺ chelator, and CaM antagonists. Under low-K⁺ stress, reactive oxygen species (ROS) accumulated, and the activity of antioxidant enzymes, NAD kinase (NADK), and NADP phosphatase (NADPase) decreased. This indicates that antioxidant enzymes, NADK, and NADPase might be downstream target proteins in the Ca²⁺-CaM signaling pathway, which facilitates K⁺ uptake in plant roots by mediating ROS homeostasis under low-K⁺ stress. Moreover, the expression of NtCNGC3, NtCNGC10, K⁺ channel genes, and transporter genes was significantly downregulated in blocker-treated, chelator-treated, and antagonist-treated plant roots in the low K⁺ treatment, suggesting that the Ca²⁺-CaM signaling pathway may mediate K⁺ uptake by regulating the expression of these genes. Overall, this study shows that the Ca²⁺-CaM signaling pathway promotes K⁺ absorption by regulating ROS homeostasis and the expression of K⁺ uptake-related genes in plant roots under low-K⁺ stress.