AUTHOR=Zhai Zhiyang , Keereetaweep Jantana , Liu Hui , Feil Regina , Lunn John E. , Shanklin John 

TITLE=Expression of a Bacterial Trehalose-6-phosphate Synthase otsA Increases Oil Accumulation in Plant Seeds and Vegetative Tissues

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.656962

DOI=10.3389/fpls.2021.656962

ISSN=1664-462X

ABSTRACT=<p>We previously demonstrated that exogenous trehalose 6-phosphate (T6P) treatment stabilized WRINKLED1 (WRI1), a master transcriptional regulator of fatty acid (FA) synthesis and increased total FA content in <italic>Brassica napus</italic> (<italic>B. napus</italic>) embryo suspension cell culture. Here, we explore <italic>Arabidopsis</italic> lines heterologously expressing the <italic>Escherichia coli</italic> T6P synthase (otsA) or T6P phosphatase (otsB) to refine our understanding regarding the role of T6P in regulating fatty acid synthesis both in seeds and vegetative tissues. <italic>Arabidopsis</italic> 35S:<italic>otsA</italic> transgenic seeds showed an increase of 13% in fatty acid content compared to those of wild type (WT), while seeds of 35:<italic>otsB</italic> transgenic seeds showed a reduction of 12% in fatty acid content compared to WT. Expression of otsB significantly reduced the level of WRI1 and expression of its target genes in developing seeds. Like <italic>Arabidopsis</italic> seeds constitutively expressing otsA, transient expression of otsA in <italic>Nicotiana benthamiana</italic> leaves resulted in strongly elevated levels of T6P. This was accompanied by an increase of 29% in <italic>de novo</italic> fatty acid synthesis rate, a 2.3-fold increase in triacylglycerol (TAG) and a 20% increase in total fatty acid content relative to empty vector (EV) controls. Taken together, these data support the heterologous expression of otsA as an approach to increasing TAG accumulation in plant seeds and vegetative tissues.</p>