AUTHOR=Ma Yaping , Xie Yun , Ha Rong , Cao Bing , Song Lihua 

TITLE=Effects of Elevated CO2 on Photosynthetic Accumulation, Sucrose Metabolism-Related Enzymes, and Genes Identification in Goji Berry (Lycium barbarum L.)

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.643555

DOI=10.3389/fpls.2021.643555

ISSN=1664-462X

ABSTRACT=<p>Goji berry (<italic>Lycium barbarum</italic> L.) exposure to elevated CO<sub>2</sub> (eCO<sub>2</sub>) for long periods reduces their sugar and secondary metabolite contents. However, sugar accumulation in fruit depends on photosynthesis and photoassimilate partitioning. This study aimed to explore photosynthesis, sugar content, and sucrose metabolism-related enzyme activities in goji berry leaves and fruits under ambient and eCO<sub>2</sub> levels, and identify the genes encoding <italic>L. barbarum</italic> acid invertase (<italic>LBAI</italic>), <italic>L. barbarum</italic> sucrose synthase (<italic>LBSS</italic>), <italic>L. barbarum</italic> sucrose phosphate synthase (<italic>LBSPS</italic>), and <italic>L. barbarum</italic> neutral invertase (<italic>LBNI</italic>), based on transcriptome profiling. Further, the characterization of four identified genes was analyzed including subcellular localization and expression patterns. In plants grown under eCO<sub>2</sub> for 90 or 120 days, the expression of the above-mentioned genes changed significantly as the photosynthetic rate increased. In addition, leaf and fruit sugar contents decreased, and the activities of four sucrose metabolism-related enzymes increased in leaves, while acid and neutral invertase increased in fruits. Protein sequence analysis demonstrated that <italic>LBAI</italic> and <italic>LBNI</italic> contain a conservative structure domain belonging to the glycosyl hydrolases (Glyco_hydro) family, and both <italic>LBSS</italic> and <italic>LBSPS</italic> belonging to the sucrose synthase (Sucrose_synth) and glycosyltransferase (Glycos_transf) family. Subcellular localization analysis showed that <italic>LBAI</italic>, <italic>LBNI</italic>, and <italic>LBSS</italic> were all located in the nucleus, plasma membrane, and cytoplasm, while <italic>LBSPS</italic> was located in the plasma membrane. The expressions of <italic>LBAI</italic>, <italic>LBSPS</italic>, and <italic>LBNI</italic> were high in the stems, whereas <italic>LBSS</italic> was predominantly expressed in the fruits. Our findings provide fundamental data on photosynthesis and sugar accumulation trends in goji berries under eCO<sub>2</sub> exposure.</p>