AUTHOR=Soler-Garzón Alvaro , Oladzad Atena , Beaver James , Beebe Stephen , Lee Rian , Lobaton Juan David , Macea Eliana , McClean Phillip , Raatz Bodo , Rosas Juan Carlos , Song Qijian , Miklas Phillip N. 

TITLE=NAC Candidate Gene Marker for bgm-1 and Interaction With QTL for Resistance to Bean Golden Yellow Mosaic Virus in Common Bean

JOURNAL=Frontiers in Plant Science

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.628443

DOI=10.3389/fpls.2021.628443

ISSN=1664-462X

ABSTRACT=<p>Genetic resistance is the primary means for control of <italic>Bean golden yellow mosaic virus</italic> (BGYMV) in common bean (<italic>Phaseolus vulgaris L.</italic>). Breeding for resistance is difficult because of sporadic and uneven infection across field nurseries. We sought to facilitate breeding for BGYMV resistance by improving marker-assisted selection (MAS) for the recessive <italic>bgm-1</italic> gene and identifying and developing MAS for quantitative trait loci (QTL) conditioning resistance. Genetic linkage mapping in two recombinant inbred line populations and genome-wide association study (GWAS) in a large breeding population and two diversity panels revealed a candidate gene for <italic>bgm-1</italic> and three QTL BGY4.1, BGY7.1, and BGY8.1 on independent chromosomes. A mutation (5 bp deletion) in a NAC (No Apical Meristem) domain transcriptional regulator superfamily protein gene <italic>Phvul.003G027100</italic> on chromosome Pv03 corresponded with the recessive <italic>bgm-1</italic> resistance allele. The five bp deletion in exon 2 starting at 20 bp (Pv03: 2,601,582) is expected to cause a stop codon at codon 23 (Pv03: 2,601,625), disrupting further translation of the gene. A T<sub><italic>m</italic></sub>-shift assay marker named PvNAC1 was developed to track <italic>bgm-1</italic>. PvNAC1 corresponded with <italic>bgm-1</italic> across ∼1,000 lines which trace <italic>bgm-1</italic> back to a single landrace “Garrapato” from Mexico. BGY8.1 has no effect on its own but exhibited a major effect when combined with <italic>bgm-1</italic>. BGY4.1 and BGY7.1 acted additively, and they enhanced the level of resistance when combined with <italic>bgm-1</italic>. T<sub><italic>m</italic></sub>-shift assay markers were generated for MAS of the QTL, but their effectiveness requires further validation.</p>