AUTHOR=Liu Qing , Xu Jinke , Zhu Yunhua , Mo Yuxing , Yao Xue-Feng , Wang Ruozhong , Ku Wenzhen , Huang Zhigang , Xia Shitou , Tong Jianhua , Huang Chao , Su Yi , Lin Wanhuang , Peng Keqin , Liu Chun-Ming , Xiao Langtao 

TITLE=The Copy Number Variation of OsMTD1 Regulates Rice Plant Architecture

JOURNAL=Frontiers in Plant Science

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.620282

DOI=10.3389/fpls.2020.620282

ISSN=1664-462X

ABSTRACT=<p>Copy number variation (CNV) may have phenotypic effects by altering the expression level of the gene(s) or regulatory element(s) contained. It is believed that CNVs play pivotal roles in controlling plant architecture and other traits in plant. However, the effects of CNV contributing to special traits remain largely unknown. Here we report a CNV involved in rice architecture by modulating tiller number and leaf angle. In the genome of <italic>Oryza sativa</italic> ssp. <italic>japonica</italic> cv. Nipponbare, we found a locus <italic>Loc_Os08g34249</italic> is derived from a 13,002-bp tandem duplication in the nearby region of <italic>OsMTD1</italic>, a gene regulating tillering in rice. Further survey of 230 rice cultivars showed that the duplication occurred in only 13 <italic>japonica</italic> rice cultivars. Phenotypic investigation indicated that this CNV region may contribute to tiller number. Moreover, we revealed that <italic>OsMTD1</italic> not only influences rice tiller number and leaf angle, but also represses <italic>pri-miR156f</italic> transcription in the CNV region. Intriguingly, this CNV performs function through both the dosage and position effects on <italic>OsMTD1</italic> and <italic>pri-miR156f</italic>. Thus, our work identified a CNV and revealed a molecular regulatory basis for its effects on plant architecture, implying this CNV may possess importance and application potential in molecular breeding in rice.</p>