AUTHOR=Li Qiao , Pan Zhifen , Gao Yuan , Li Tao , Liang Junjun , Zhang Zijin , Zhang Haili , Deng Guangbing , Long Hai , Yu Maoqun 

TITLE=Quantitative Trait Locus (QTLs) Mapping for Quality Traits of Wheat Based on High Density Genetic Map Combined With Bulked Segregant Analysis RNA-seq (BSR-Seq) Indicates That the Basic 7S Globulin Gene Is Related to Falling Number

JOURNAL=Frontiers in Plant Science

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.600788

DOI=10.3389/fpls.2020.600788

ISSN=1664-462X

ABSTRACT=<p>Numerous quantitative trait loci (QTLs) have been identified for wheat quality; however, most are confined to low-density genetic maps. In this study, based on specific-locus amplified fragment sequencing (SLAF-seq), a high-density genetic map was constructed with 193 recombinant inbred lines derived from Chuanmai 42 and Chuanmai 39. In total, 30 QTLs with phenotypic variance explained (PVE) up to 47.99% were identified for falling number (FN), grain protein content (GPC), grain hardness (GH), and starch pasting properties across three environments. Five <italic>NAM</italic> genes closely adjacent to <italic>QGPC.cib-4A</italic> probably have effects on GPC. <italic>QGH.cib-5D</italic> was the only one detected for GH with high PVE of 33.31–47.99% across the three environments and was assumed to be related to the nearest <italic>pina-D1</italic> and <italic>pinb-D1</italic>genes. Three QTLs were identified for FN in at least two environments, of which <italic>QFN.cib-3D</italic> had relatively higher PVE of 16.58–25.74%. The positive effect of <italic>QFN.cib-3D</italic> for high FN was verified in a double-haploid population derived from Chuanmai 42 <bold>×</bold> Kechengmai 4. The combination of these QTLs has a considerable effect on increasing FN. The transcript levels of <italic>Basic 7S globulin</italic> and <italic>Basic 7S globulin 2</italic> in <italic>QFN.cib-3D</italic> were significantly different between low FN and high FN bulks, as observed through bulk segregant RNA-seq (BSR). These QTLs and candidate genes based on the high-density genetic map would be beneficial for further understanding of the genetic mechanism of quality traits and molecular breeding of wheat.</p>