AUTHOR=Burman Naini , Chandran Divya , Khurana Jitendra P. TITLE=A Rapid and Highly Efficient Method for Transient Gene Expression in Rice Plants JOURNAL=Frontiers in Plant Science VOLUME=11 YEAR=2020 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.584011 DOI=10.3389/fpls.2020.584011 ISSN=1664-462X ABSTRACT=
Rice is the model plant system for monocots and the sequencing of its genome has led to the identification of a vast array of genes for characterization. The tedious and time-consuming effort of raising rice transgenics has significantly delayed the pace of rice research. The lack of highly efficient transient assay protocol for rice has only added to the woes which could have otherwise helped in rapid deciphering of the functions of genes. Here, we describe a technique for efficient transient gene expression in rice seedlings. It makes use of co-cultivation of 6-day-old rice seedlings with