AUTHOR=Cao Chu Uyen , Kumar Sandeep , Sigmund Amy , Johnson Kari , Li Yinghong , Vongdeuane Pamila , Jones Todd J. 

TITLE=Genotype-Independent Transformation and Genome Editing of Brassica napus Using a Novel Explant Material

JOURNAL=Frontiers in Plant Science

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.579524

DOI=10.3389/fpls.2020.579524

ISSN=1664-462X

ABSTRACT=<p><italic>Agrobacterium</italic>-mediated transformation of canola (<italic>Brassica napus</italic>) via hypocotyl segments has been a commonly used method for the past 30 years. While the hypocotyl-based method is well-established, it is not readily adapted to elite germplasm and the prolonged process is not ideal for a production transformation setting. We developed an <italic>Agrobacterium</italic>-mediated transformation method based on epicotyl and higher stem (internodal) segments that is efficient, rapid and amenable for high-throughput transformation and genome editing. The method has been successfully implemented in multiple canola genotypes. The method appears to be genotype-independent, with varying transformation efficiencies. Internodal segment transformation was used to generate transgenic events as well as CRISPR-Cas9-mediated frameshift gene knockouts.</p>