AUTHOR=Huang XinHao , Zhu Fan , He ZhiXiang , Chen XiaoYong , Wang GuangJun , Liu MengShan , Xu HongYang 

TITLE=Photosynthesis Performance and Antioxidative Enzymes Response of Melia azedarach and Ligustrum lucidum Plants Under Pb–Zn Mine Tailing Conditions

JOURNAL=Frontiers in Plant Science

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.571157

DOI=10.3389/fpls.2020.571157

ISSN=1664-462X

ABSTRACT=<p>Lead–zinc (Pb–Zn) mine tailings pose a great risk to the natural environment and human health because of their high toxicity. In this study, the responses of photosynthesis, chlorophyll fluorescence, and antioxidative enzyme of <italic>Melia azedarach</italic> and <italic>Ligustrum lucidum</italic> in the soil contaminated by Pb–Zn mine tailings were investigated. Results showed that Pb–Zn mine tailings significantly reduced net photosynthetic rates and leaf photosynthetic pigment content of both trees, and the reduction of net photosynthetic rates was mainly caused by their biochemical limitation (BL). The chlorophyll fluorescence parameters from Pb–Zn tailing stressed leaves indicated that Pb–Zn tailings affected PSII activity which was evident from the change values of energy fluxes per reaction center (RC): probability that an electron moves further than Q<sub>A</sub><sup>−</sup> (ET<sub>O</sub>/TR<sub>O</sub>), maximum quantum yield for primary photochemistry (TR<sub>O</sub>/ABS), the density of PSII RC per excited cross-section (RC/CS<sub>O</sub>), the absorption of antenna chlorophylls per PSII RC (ABS/RC), and the turnover number of Q<sub>A</sub> reduction events (N). Pb–Zn mine tailings also affected the oxidation and reduction of PSI, which resulted in a great increase of reactive oxygen species (ROS) contents and then stimulated the rate of lipid peroxidation. Both trees exhibited certain antioxidative defense mechanisms as elevated superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities, then declined under high level of Pb–Zn tailing treatment. Comparatively, <italic>L. lucidum</italic> showed less extent effect on photosynthesis and higher antioxidative enzyme activities than <italic>M. azedarach</italic>; thus <italic>L. lucidum</italic> was more tolerant than <italic>M. azedarach</italic> at least under the described Pb–Zn tailing treatment. These results indicate that the effect of Pb–Zn mine tailings on photosynthesis performance mainly related to imbalance of the PSII activity and PSI redox state in both trees. We propose that <italic>M. azedarach</italic> and <italic>L. lucidum</italic> could relieve the oxidative stress for phytoremediation under the appropriate Pb–Zn mine tailing content.</p>