AUTHOR=Chen Shu , Huang Huai-An , Chen Jian-Hui , Fu Cheng-Cheng , Zhan Peng-Lin , Ke Shan-Wen , Zhang Xiang-Qian , Zhong Tian-Xiu , Xie Xin-Ming 

TITLE=SgRVE6, a LHY-CCA1-Like Transcription Factor From Fine-Stem Stylo, Upregulates NB-LRR Gene Expression and Enhances Cold Tolerance in Tobacco

JOURNAL=Frontiers in Plant Science

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.01276

DOI=10.3389/fpls.2020.01276

ISSN=1664-462X

ABSTRACT=<p><italic>Stylosanthes</italic> species are economically important tropical and subtropical forage legumes which are generally vulnerable to chilling and frost. Fine-stem stylo (<italic>S. guianensis</italic> var. <italic>intermedia</italic>) has the most superior cold tolerance among all stylo species. A <italic>REVEILLE</italic> (<italic>RVE</italic>) gene, <italic>SgRVE6</italic>, was cloned from fine-stem stylo. Bioinformatic analysis suggests that <italic>SgRVE6</italic> encodes a transcription factor of 292 amino acid residues, which belongs to the LATE ELONGATED HYPOCOTYL/CIRCADIAN CLOCK ASSOCIATED 1-LIKE (LCL) subgroup of RVE family and contains a SHAQKYF-class MYB domain and a LCL domain. <italic>SgRVE6</italic> is universally expressed in root, stem and leaf tissues of fine-stem stylo and is rapidly up-regulated in all tested tissues under cold stress. Over-expressing <italic>SgRVE6</italic> affects expression of 21 circadian clock genes, up-regulates expression of 6 nucleotide binding domain leucine-rich repeats (NB-LRR) encoding genes associated with tobacco cold tolerance, improves physiological responses to low temperature, and endows the transgenic tobaccos with higher tolerance to cold stress. This is the first time a study investigates the biological function of RVE6 in cold responses of plant species.</p>