AUTHOR=Liu Yuling , Wang Xiuyuan , Wei Yangyang , Liu Zhen , Lu Quanwei , Liu Fang , Zhang Tao , Peng Renhai 

TITLE=Chromosome Painting Based on Bulked Oligonucleotides in Cotton

JOURNAL=Frontiers in Plant Science

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00802

DOI=10.3389/fpls.2020.00802

ISSN=1664-462X

ABSTRACT=<p>Chromosome painting is one of the key technologies in cytogenetic research, which can accurately identify chromosomes or chromosome regions. Oligonucleotide (oligo) probes designed based on genome sequences have both flexibility and specificity, which would be ideal probes for fluorescence <italic>in situ</italic> hybridization (FISH) analysis of genome structure. In this study, the bulked oligos of the two arms of chromosome seven of cotton were developed based on the genome sequence of <italic>Gossypium raimondii</italic> (DD, 2<italic>n</italic> = 2× = 26), and each arm contains 12,544 oligos. Chromosome seven was easily identified in both D genome and AD genome cotton species using the bulked chromosome-specific painting probes. Together with 45S ribosomal DNA (rDNA) probe, the chromosome-specific painting probe was also successfully used to correct the chromosomal localization of 45S rDNA in <italic>G. raimondii</italic>. The study reveals that bulked oligos specific to a chromosome is a useful tool for chromosome painting in cotton.</p>