AUTHOR=Dell’Aquila Gianluca , Zauner Stefan , Heimerl Thomas , Kahnt Jörg , Samel-Gondesen Vera , Runge Simon , Hempel Franziska , Maier Uwe G. 

TITLE=Mobilization and Cellular Distribution of Phosphate in the Diatom Phaeodactylum tricornutum

JOURNAL=Frontiers in Plant Science

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00579

DOI=10.3389/fpls.2020.00579

ISSN=1664-462X

ABSTRACT=<p>Unicellular organisms that live in marine environments must cope with considerable fluctuations in the availability of inorganic phosphate (P<sub>i</sub>). Here, we investigated the extracellular P<sub>i</sub> concentration-dependent expression, as well as the intracellular or extracellular localization, of phosphatases and phosphate transporters of the diatom <italic>Phaeodactylum tricornutum</italic>. We identified P<sub>i</sub>-regulated plasma membrane-localized, ER-localized, and secreted phosphatases, in addition to plasma membrane-localized, vacuolar membrane-localized, and plastid-surrounding membrane-localized phosphate transporters that were also regulated in a P<sub>i</sub> concentration-dependent manner. These studies not only add further knowledge to already existing transcriptomic data, but also highlight the capacity of the diatom to distribute P<sub>i</sub> intracellularly and to mobilize P<sub>i</sub> from extracellular and intracellular resources.</p>