AUTHOR=Zeng Rui-Zhen , Zhu Jiao , Xu Shi-Ying , Du Guo-Hui , Guo He-Rong , Chen Jianjun , Zhang Zhi-Sheng , Xie Li 

TITLE=Unreduced Male Gamete Formation in Cymbidium and Its Use for Developing Sexual Polyploid Cultivars

JOURNAL=Frontiers in Plant Science

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00558

DOI=10.3389/fpls.2020.00558

ISSN=1664-462X

ABSTRACT=<p>Polyploidy plays an important role in crop improvement. Polyploid plants, particularly those produced through unreduced gametes (2<italic>n</italic> gametes), show increased organ size, improved buffering capacity for deleterious mutations, and enhanced heterozygosity and heterosis. Induced polyploidy has been widely used for improving floriculture crops, however, there are few reported sexual polyploid plants in the floriculture industry. This study evaluated nine cultivars of <italic>Cymbidium</italic> Swartz and discovered that 2<italic>n</italic> male gametes occurred in this important orchid. Depending on cultivars, 2<italic>n</italic> male gamete formation frequencies varied from 0.15 to 4.03%. Interspecific hybrids generally produced more 2<italic>n</italic> male gametes than traditional cultivars. To generate sexual polyploid plants, seven pairs of crosses were made, which produced five triploid and two tetraploid hybrids. Two triploid hybrids were evaluated for <italic>in vitro</italic> regeneration and growth characteristics. Compared to the diploid parents, the triploids were more easily regenerated through rhizomes or protocorms, and regenerated plants had improved survival rates after transplanting to the greenhouse. Furthermore, the sexual polyploid plants had more compact growth style, produced fragrant flowers, and demonstrated heterosis in plant growth. Through this study, a reliable protocol for selection of appropriate parents for 2<italic>n</italic> gamete production, ploidy level evaluation, <italic>in vitro</italic> culture of polyploid progenies, and development of new polyploid cultivars was established. Our study with <italic>Cymbidium</italic> suggests that the use of 2<italic>n</italic> gametes is a viable approach for improving floriculture crops.</p>