AUTHOR=Ma Jingjing , Yang Suxin , Wang Dongmei , Tang Kuanqiang , Feng Xing Xing , Feng Xian Zhong 

TITLE=Genetic Mapping of a Light-Dependent Lesion Mimic Mutant Reveals the Function of Coproporphyrinogen III Oxidase Homolog in Soybean

JOURNAL=Frontiers in Plant Science

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00557

DOI=10.3389/fpls.2020.00557

ISSN=1664-462X

ABSTRACT=<p>Lesion mimic mutants provide ideal genetic materials for elucidating the molecular mechanism of cell death and disease resistance. Here, we isolated a <italic>Glycine max lesion mimic mutant 2-1</italic> (<italic>Gmlmm2-1</italic>), which displayed a light-dependent cell death phenotype. Map-based cloning revealed that <italic>GmLMM2</italic> encods a coproporphyrinogen III oxidase and participates in tetrapyrrole biosynthesis. Knockout of <italic>GmLMM2</italic> led to necrotic spots on developing leaves of CRISPR/Cas9 induced mutants. The <italic>GmLMM2</italic> defect decreased the chlorophyll content by disrupting tetrapyrrole biosynthesis and enhanced resistance to <italic>Phytophthora sojae</italic>. These results suggested that <italic>GmLMM2</italic> gene played an important role in the biosynthesis of tetrapyrrole and light-dependent defense in soybeans.</p>