AUTHOR=Fu Can , Liu Xiaxia , Li Xuezhi , Huo Panfei , Ge Jingjing , Hou Yanfei , Yang Wenwen , Zhang Jingxia , Zhang Limin , Zhao Dazhong , Ma Changle , Liu Jian 

TITLE=BRF Negatively Regulates Thermotolerance Defect of fes1a in Arabidopsis

JOURNAL=Frontiers in Plant Science

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00171

DOI=10.3389/fpls.2020.00171

ISSN=1664-462X

ABSTRACT=<p>FES1A is a heat shock protein 70 binding protein. Mutation of <italic>FES1A</italic> leads to a defect in thermotolerance of <italic>Arabidopsis</italic>; however, independent <italic>fes1a</italic> mutants exhibit a range in the extent of thermosensitivity. Here, we found that <italic>BRF2</italic>, a gene adjacent to <italic>FES1A</italic> and encoding a component of transcription factor IIIB, affects the thermosensitivity of <italic>fes1a</italic> mutants. Knockout of <italic>BRF2</italic> suppressed <italic>fes1a</italic> thermosensitivity, while overexpression of <italic>BRF2</italic> increased thermosensitivity of <italic>fes1a.</italic> BRF2 in <italic>fes1a</italic> mutants regulates the transcriptional strength of RNA Polymerase II and accumulation of heat shock proteins and eventually affects the thermotolerance of <italic>fes1a</italic>. There is a cross-talking between RNA Pol III and Pol II. The cross-talking is initiated by <italic>BRF</italic>, magnified by the mutation of <italic>FES1A,</italic> and finally has an effect on thermotolerance.</p>