AUTHOR=Sanyal Sibaji K. , Kanwar Poonam , Fernandes Joel Lars , Mahiwal Swati , Yadav Akhilesh K. , Samtani Harsha , Srivastava Ashish K. , Suprasanna Penna , Pandey Girdhar K. 

TITLE=Arabidopsis Mitochondrial Voltage-Dependent Anion Channels Are Involved in Maintaining Reactive Oxygen Species Homeostasis, Oxidative and Salt Stress Tolerance in Yeast

JOURNAL=Frontiers in Plant Science

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00050

DOI=10.3389/fpls.2020.00050

ISSN=1664-462X

ABSTRACT=<p>Voltage-dependent anion channels (VDACs) are conserved proteins of the mitochondria. We have functionally compared <italic>Arabidopsis</italic> VDACs using <italic>Saccharomyces cerevisiae Δpor1</italic> and M3 yeast system. VDAC (1, 2, and 4) were able to restore <italic>Δpor1</italic> growth in elevated temperature, in oxidative and salt stresses, whereas VDAC3 only partially rescued <italic>Δpor1</italic> in these conditions. The ectopic expression of <italic>VDAC</italic> (1, 2, 3, and 4) in mutant yeast recapitulated the mitochondrial membrane potential thus, enabled it to maintain reactive oxygen species homeostasis. Overexpression of these VDACs (<italic>AtVDACs)</italic> in M3 strain did not display any synergistic or antagonistic activity with the native yeast VDAC1 (ScVDAC1). Collectively, our data suggest that <italic>Arabidopsis</italic> VDACs are involved in regulating respiration, reactive oxygen species homeostasis, and stress tolerance in yeast.</p>