AUTHOR=Escudero Viviana , Abreu Isidro , del Sastre Eric , Tejada-Jiménez Manuel , Larue Camille , Novoa-Aponte Lorena , Castillo-González Jorge , Wen Jiangqi , Mysore Kirankumar S. , Abadía Javier , Argüello José M. , Castillo-Michel Hiram , Álvarez-Fernández Ana , Imperial Juan , González-Guerrero Manuel TITLE=Nicotianamine Synthase 2 Is Required for Symbiotic Nitrogen Fixation in Medicago truncatula Nodules JOURNAL=Frontiers in Plant Science VOLUME=10 YEAR=2020 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01780 DOI=10.3389/fpls.2019.01780 ISSN=1664-462X ABSTRACT=
Symbiotic nitrogen fixation carried out by the interaction between legumes and diazotrophic bacteria known as rhizobia requires relatively large levels of transition metals. These elements are cofactors of many key enzymes involved in this process. Metallic micronutrients are obtained from soil by the roots and directed to sink organs by the vasculature, in a process mediated by a number of metal transporters and small organic molecules that facilitate metal delivery in the plant fluids. Among the later, nicotianamine is one of the most important. Synthesized by nicotianamine synthases (NAS), this molecule forms metal complexes participating in intracellular metal homeostasis and long-distance metal trafficking. Here we characterized the