AUTHOR=Zhuang Yamei , Wang Congpeng , Zhang Yang , Chen Sihui , Wang Dian , Liu Qing , Zhou Gongke , Chai Guohua 

TITLE=Overexpression of PdC3H17 Confers Tolerance to Drought Stress Depending on Its CCCH Domain in Populus

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01748

DOI=10.3389/fpls.2019.01748

ISSN=1664-462X

ABSTRACT=<p>Plant CCCH zinc finger proteins control growth, development, and stress responses mainly at the post-transcriptional level. Currently, limited reports are available about the roles of plant CCCH proteins in drought tolerance. In this study, we provided evidence showing that <italic>Pd</italic>C3H17 from <italic>Populus deltoides × P. euramericana</italic> involves drought tolerance and response. Overexpression of <italic>PdC3H17</italic> in poplar caused dwarf, resulted in higher stem water potential, and showed increased photosynthetic and ROS-scavenging abilities, thereby enhancing tolerance to drought stress, compared to controls. Accordingly, after drought treatment the stem elongation and thickening rates of these overexpression lines were higher than those of the controls. However, overexpression of the coding region excluding the CCCH domain of <italic>Pd</italic>C3H17 roughly exhibited WT-like physiological and drought-resistant phenotypes, indicating the requirement of the CCCH domain for <italic>Pd</italic>C3H17 controlling these processes. In addition, N-terminal sequence of <italic>Pd</italic>C3H17 was found to possess transcriptional activity ability in yeast cells. Together, our results suggest that <italic>Pd</italic>C3H17 may depend on its CCCH domain to control drought tolerance in <italic>Populus</italic>.</p>