AUTHOR=Wei Xiaopeng , Mao Linchun , Lu Wenjing , Wei Xiaobo , Han  Xueyuan , Guan Weiliang , Yang Yajie , Zha Meng , Xu Changjie , Luo Zisheng 

TITLE=Three Transcription Activators of ABA Signaling Positively Regulate Suberin Monomer Synthesis by Activating Cytochrome P450 CYP86A1 in Kiwifruit

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01650

DOI=10.3389/fpls.2019.01650

ISSN=1664-462X

ABSTRACT=<p>Wound attack stimulates accumulation of abscisic acid (ABA) that activates a number of genes associated with wound suberization of plants. Cytochrome P450 fatty acid ω-hydroxylase CYP86A1 catalyzes ω-hydroxylation of fatty acids to form the ω-functionalized monomers that play a pivotal role in suberin synthesis. However, the transcriptional regulation of ABA signaling on <italic>AchnCYP86A1</italic> has not been characterized in kiwifruit. In this study, <italic>AchnCYP86A1</italic>, a kiwifruit homolog of <italic>Arabidopsis AtCYP86A1</italic>, was isolated. <italic>AchnCYP86A1</italic>-overexpressed <italic>N. benthamiana</italic> leaves displayed that the AchnCYP86A1 functioned as a fatty acid ω-hydroxylase associated with synthesis of suberin monomer. The regulatory function of three transcription factors (TFs, including AchnMYC2, AchnMYB41 and AchnMYB107) on <italic>AchnCYP86A1</italic> was identified. All the three TFs were localized in nucleus and could individually interact with <italic>AchnCYP86A1</italic> promoter to activate gene expression in yeast one-hybrid and dual-luciferase assays. The findings were further demonstrated in transient overexpressed <italic>N. benthamiana</italic>, in which all TFs notably elevated the expression of aliphatic synthesis genes including <italic>CYP86A1</italic> and the accumulation of ω-hydroxyacids, α, ω-diacids, fatty acids and primary alcohols. Moreover, exogenous ABA induced the expression of <italic>AchnMYC2</italic>, <italic>AchnMYB41</italic> and <italic>AchnMYB107</italic> that promoted <italic>AchnCYP86A1</italic> involving in suberin monomer formation. Contrary to the inductive effects of ABA, however, fluridone (an inhibitor of ABA biosynthesis) inhibited the three TFs expression and suberin monomer formation. These results indicate that AchnMYC2, AchnMYB41 and AchnMYB107 positively regulate suberin monomer synthesis by activating <italic>AchnCYP86A1</italic> promoter in response to ABA.</p>