AUTHOR=Bleackley Mark R. , Samuel Monisha , Garcia-Ceron Donovan , McKenna James A. , Lowe Rohan G. T. , Pathan Mohashin , Zhao Kening , Ang Ching-Seng , Mathivanan Suresh , Anderson Marilyn A. 

TITLE=Extracellular Vesicles From the Cotton Pathogen Fusarium oxysporum f. sp. vasinfectum Induce a Phytotoxic Response in Plants

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01610

DOI=10.3389/fpls.2019.01610

ISSN=1664-462X

ABSTRACT=<p>Extracellular vesicles (EVs) represent a system for the coordinated secretion of a variety of molecular cargo including proteins, lipids, nucleic acids, and metabolites. They have an essential role in intercellular communication in multicellular organisms and have more recently been implicated in host-pathogen interactions. Study of the role for EVs in fungal biology has focused on pathogenic yeasts that are major pathogens in humans. In this study we have expanded the investigation of fungal EVs to plant pathogens, specifically the major cotton pathogen <italic>Fusarium oxysporum</italic> f. sp. <italic>vasinfectum</italic>. EVs isolated from <italic>F. oxysporum</italic> f. sp. <italic>vasinfectum</italic> culture medium have a morphology and size distribution similar to EVs from yeasts such as <italic>Candida albicans</italic> and <italic>Cryptococcus neoformans</italic>. A unique feature of the EVs from <italic>F. oxysporum</italic> f. sp. <italic>vasinfectum</italic> is their purple color, which is predicted to arise from a napthoquinone pigment being packaged into the EVs. Proteomic analysis of <italic>F. oxysporum</italic> f. sp. <italic>vasinfectum</italic> EVs revealed that they are enriched in proteins that function in synthesis of polyketides as well as proteases and proteins that function in basic cellular processes. Infiltration of <italic>F. oxysporum</italic> f. sp. <italic>vasinfectum</italic> EVs into the leaves of cotton or <italic>N. benthamiana</italic> plants led to a phytotoxic response. These observations lead to the hypothesis that <italic>F. oxysporum</italic> f. sp. <italic>vasinfectum</italic> EVs are likely to play a crucial role in the infection process.</p>