AUTHOR=Wang Zhongtang , Zhang Zhong , Tang Haixia , Zhang Qiong , Zhou Guangfang , Li Xingang 

TITLE=High-Density Genetic Map Construction and QTL Mapping of Leaf and Needling Traits in Ziziphus jujuba Mill

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01424

DOI=10.3389/fpls.2019.01424

ISSN=1664-462X

ABSTRACT=<p>The Chinese jujube (<italic>Ziziphus jujuba</italic> Mill., 2<italic>n</italic> = 2<italic>x</italic> = 24), one of the most popular fruit trees in Asia, is widely cultivated and utilized in China, where it is traditionally consumed as both a fresh and dried food resource. A high-density genetic map can provide the necessary framework for quantitative trait loci (QTL) analyses and map-based gene cloning and molecular breeding. In this study, we constructed a new high-density genetic linkage map <italic>via</italic> a genotyping-by-sequencing approach. For the consensus linkage map, a total of 3,792 markers spanning 2,167.5 cM were mapped onto 12 linkage groups, with an average marker interval distance of 0.358 cM. The genetic map anchored 301 Mb (85.7%) of scaffolds from the sequenced <italic>Z</italic>. <italic>jujuba</italic> “Junzao” genome. Based on this genetic map, 30 potential QTLs were detected, including 27 QTLs for leaf traits and 3 QTLs for needling length. This high-density genetic map and the identified QTLs for relevant agronomic traits lay the groundwork for functional genetic mapping, map-based cloning, and marker-assisted selection in jujube.</p>