Oscar Alejandro Pérez-Escobar1,2*†
Eve Lucas1†
Carlos Jaramillo3,4†
Alexandre Monro1
Sarah K. Morris1†
Diego Bogarín5Deborah Greer1,6
Steven Dodsworth7José Aguilar-Cano8Andrea Sanchez Meseguer9
Alexandre Antonelli1,2- 1Comparative Plant and Fungal Biology Department, Royal Botanic Gardens Kew, London, United Kingdom
- 2Gothenburg Global Biodiversity Centre, Gothenburg, Sweden
- 3Smithsonian Tropical Research Institute, Panama, Panama
- 4ISEM, U. Montpellier, CNRS, EPHE, IRD, Montpellier, France
- 5Universidad de Costa Rica, Jardín Botánico Lankester, Cartago, Costa Rica
- 6Department of Environment, Food and Rural Affairs, London, United Kingdom
- 7School of Life Sciences, University of Bedfordshire, Luton, United Kingdom
- 8Research Institute Alexander von Humboldt, Bogota, Colombia
- 9Real Jardín Botánico de Madrid (RJB-CSIC), Madrid, Spain
Extremely high levels of plant diversity in the American tropics are derived from multiple interactions between biotic and abiotic factors. Previous studies have focused on macro-evolutionary dynamics of the Tropical Andes, Amazonia, and Brazil’s Cerrado and Atlantic forests during the last decade. Yet, other equally important Neotropical biodiversity hotspots have been severely neglected. This is particularly true for the Chocó region on the north-western coast of South and Central America. This geologically complex region is Earth’s ninth most biodiverse hotspot, hosting approximately 3% of all known plant species. Here, we test Gentry’s [1982a,b] hypothesis of a northern Andean-Central American Pleistocene origin of the Chocoan flora using phylogenetic reconstructions of representative plant lineages in the American tropics. We show that plant diversity in the Chocó is derived mostly from Andean immigrants. Contributions from more distant biogeographical areas also exist but are fewer. We also identify a strong floristic connection between the Chocó and Central America, revealed by multiple migrations into the Chocó during the last 5 Ma. The dated phylogenetic reconstructions suggest a Plio-Pleistocene onset of the extant Chocó flora. Taken together, these results support to a limited extend Gentry’s hypothesis of a Pleistocene origin and of a compound assembly of the Chocoan biodiversity hotspot. Strong Central American–Chocoan floristic affinity may be partly explained by the accretion of a land mass derived from the Caribbean plate to north-western South America. Additional densely sampled phylogenies of Chocoan lineages also well represented across the Neotropics could enlighten the role of land mass movements through time in the assembly of floras in Neotropical biodiversity hotspots.
Introduction
“Geologically the Chocó represents a recent emergence formed as a part of the main Andean uplift, perhaps only in the mid-Pleistocene” (Gentry, 1982b).
The American tropics (a.k.a. the Neotropical realm) extends from central Mexico to southern South America including the Caribbean (Antonelli et al., 2018a) and are home to six of the most species-rich biodiversity hotspots on Earth (Mittermeier et al., 2011). The origin of plant Neotropical megadiversity and the processes driving this diversification have been studied over the past four decades (e.g. Gentry, 1982b; Gentry, 1992; Antonelli et al., 2009, Antonelli et al., 2018b; Hoorn et al., 2010). As a result, several biotic (plant–organism interactions) and abiotic factors (e.g. climate, orogeny, and plant migration dynamics) have been posited to have influenced the diversification of plant lineages in the region (Hughes and Eastwood, 2006; Antonelli and Sanmartín, 2011a; Uribe-Convers and Tank, 2015; Lagomarsino et al., 2016; Pérez-Escobar et al., 2017a).
Historically, phylogenetic-based studies have heavily focused on a restricted subset of biogeographical regions or biodiversity hotspots within the American tropics (e.g. Amazonia, tropical Andes, Central America, Brazil’s Cerrado and Atlantic forests, and Seasonally Dry Forests) and their most prominent plant groups, including Annonaceae (Erkens et al., 2007; Pirie et al., 2018), Arecaceae (Bacon et al., 2013; Cano et al., 2018), Campanulaceae (Lagomarsino et al., 2016), Fabaceae (Richardson et al., 2001; Hughes and Eastwood, 2006; Nevado et al., 2016; Schley et al., 2018), Myrtaceae (Vasconcelos et al., 2019), and Orchidaceae (Martins et al., 2018; Pérez-Escobar et al., 2017a, Pérez-Escobar et al., 2017c). This is likely due to the possibilities of producing relatively well sampled phylogenies for such groups, built upon decades of plant collection and documentation in biogeographical regions that are relatively easy to access (Eiserhardt et al., 2017).
Biogeography, Climate and Diversity of the Chocó
Perhaps one of the least understood biodiversity hotspots in terms of species diversity and evolution in the American tropics is the Chocó biogeographic region (A.K.A. Tumbes-Chocó-Magdalena, henceforth referred as the “Chocó”; Cano et al., 2017). The Chocó is the world’s ninth most biodiverse hotspot and hosts nearly 3% (∼11,000 species) of all plant species (Christenhusz et al., 2017), including ∼2,750 endemic species, in less than 0.2% of the Earth’s land surface (Gentry, 1982b; Myers et al., 2000; Mittermeier et al., 2011). Thus, the Chocoan landscape is as rich as other megadiverse, but considerably larger biogeographical regions such as Central America. Yet, substantial knowledge gaps in the mode and tempo of evolution of the Chocoan flora still exist. These mainly stem from the notably limited availability of comprehensively sampled phylogenies of plant groups prominent in the region but also distributed across the American continent (Jaramillo, 2006). To our knowledge, no study has yet specifically attempted to disentangle the origin and drivers of diversification of the hyper diverse flora of this region using phylogenetic frameworks. Those who have sampled plant Chocoan diversity often include, at most, a handful of species that are distributed and/or restricted to the Chocó (e.g. Pérez-Escobar, 2016; Pirie et al., 2018; Canal et al., 2019; Thode et al., 2019).
Phytosociological communities in the Chocó broadly occur in 20 ecosystems types, including lowland wet forests (the dominant ecosystem), forest swamps, grasslands, coastal mangroves, and montane cloud forests (Rangel-Chui, 2011; Cano et al., 2017). The precise northern extent and delimitation of the Chocó into the Darien Gap remain contentious. Different delimitations have been employed in biogeographical studies (e.g. Gentry, 1986; Morrone, 2006; Antonelli et al., 2009; Rangel-Chui, 2011; Pérez-Escobar et al., 2017b; Cano et al., 2018), but most of these encompass the area between Central Panama (the geographical limit between Colombia and Panama) or from Southern Nicaragua to north-western Venezuela (Morrone, 2006). The southern fringes are less debated, usually marked by the wet forests of the Pacific coast of northern Ecuador (Esmeraldas Province; Figure 1).
Figure 1 Geographical extent of the Chocó biogeographic region (highlighted in blue) in Central and South America as defined by Myers (2000) and its compound geological origin. The geological extensions of the Chucunaque, Atrato, and Tumaco basins are indicated with colour coded polygons. The location of the Uramita suture and the Garrapatas fault are also provided. (A) Lowland wet forest in north-western Colombia in Purricha (Chocó Department); (B) Pre-montane gallery forest in central-western Colombia, near Queremal town (Valle del Cauca department). Photos: R. Cámara-Leret and O. Pérez]
A particular aspect of the Chocó is its extreme precipitation and humidity, which renders this region one of the “wettest places on earth” (Gentry, 1986). With a recorded annual precipitation of 3,000–11,700 mm, the Chocó stands as the second rainiest place after Cherrapunjee (India), which receives an annual mean precipitation of 12,000 mm (Banerjee et al., 2012). Detailed floristic studies led by Gentry (1986) in 0.1 ha plots in areas with different levels of rainfall in the Chocó revealed differences in species diversity correlated with precipitation. A strikingly similar correlation between species richness, individual abundancy and precipitation was found more recently to occur in palms (Copete et al., 2019). More species have been recorded from plots located in wetter areas of the Chocó than in plots situated in drier localities, even though all sites present higher individual densities compared with most other continental ecosystems around the world (Gentry, 1986).
The Intricate Climatic and Geological History of Central America and Its Influence on the Origin and Diversification of the Chocoan Flora
The geological formation of the Chocó entailed very complex processes, but is mostly the by-product of interactions between the Nazca, Cocos, and South American plates during the past 100 million years (see Borrero et al., 2012; Montes et al., 2015; Cardona et al., 2018; León et al., 2018 for extensive reviews on the tectonic evolution of the region). The Chocó is composed of two geological blocks, the Chucunaque–Atrato to the north and the Tumaco to the south (Figure 1). The Chucunaque–Atrato block belongs to the Panama microplate, which is a piece of the trailing edge of the Caribbean plate. It is an intra-arc sedimentary basin bounded by magmatic arcs, which extends from central Panama to the middle part of the Chocó region (Duque-Caro, 1990a; Duque-Caro, 1990b; Coates et al., 2004; Wegner et al., 2011; Figure 1). In other words, the geological origin of the Chocó is Central American rather than South American.
The collision of the Chucunaque–Atrato basin and its associated magmatic arc with South America commenced during the late Miocene. By ∼10 Ma, the collision had fused the Panamanian magmatic arc with the western Andes of Colombia along the Uramita suture (Montes et al., 2015; León et al., 2018). Paleogeographic reconstructions indicate that at 10 Ma, most of the Chucunaque–Atrato basin comprised shallow marine environments. Thus, by the late Miocene, in the region currently occupied by the Chocó land existed only in a) the Panamanian magmatic eastern arc that fused together with the western Andes along the Uramite suture and b) in the western arc (i.e. the Baudo range; Coates et al., 2004; Jaramillo, 2018).
A second developmental phase of the Chocó began following the onset of the collision of South America with the trailing edge of the Caribbean plate (the Panama microplate) prompting a progressive shallowing of the Chucunaque–Atrato basin, which transformed marine settings into terrestrial landscapes. The collision appears to have segmented the basin into two blocks, Chucunaque in Panama and Atrato in Colombia (Figure 1). In the Atrato basin, this shallowing probably moved from south to north, with fully terrestrial environments no older than 3.1 Ma (Duque-Caro, 1990a, Duque-Caro, 1990b), although the precise dating of the onset is still unknown and could be much younger. The southern boundary of the Atrato basin is bounded by the Garrapatas fault (Figure 1). South of Garrapatas lies the Tumaco forearc basin (Borrero et al., 2012), which covers the southern segment of the Chocó. The Tumaco forearc basin formed in an island arc setting (Borrero et al., 2012) and seems also to be part of the Caribbean plate, having collided earlier with South America compared to the Chucunaque-Atrato basin, although additional studies are needed to confirm the time of collision. Fully terrestrial environments are no older than 4.2 Ma (Borrero et al., 2012) but more precise dating of the onset of terrestrial landscape is still required as it could be younger.
Modern rainfall patterns in the Chocó are amongst the highest in the world and are driven by the Chocó jet stream centred around 5° N (Poveda and Mesa, 2000). The strength of the stream is related to temperature surface gradients between western Colombia and the tropical east Pacific (El Niño 1 + 2 geographical regions) with precipitation over the Chocó related to the high elevation of the western Cordillera, which acts as a barrier to the jet stream. The temperature gradient along the tropical Pacific intensified with the onset of the Pleistocene (Fedorov et al., 2013). Therefore, the origin of the modern Chocó jet stream may be linked with the onset of the Pleistocene.
Taken together, the geological history of the Chocó suggests that the modern landscape (i.e. fully terrestrial with high rates of precipitation) was only established by the early Pleistocene (i.e. ∼2.7 Ma). The closest source areas of plant migrants to populate this newly developed terrestrial set of ecosystems could have been the lowlands of Panama to the north, and the montane habitats of the western Cordillera to the east. In contrast, the region south of Chocó highly increased its aridity during the late Neogene, thus being an unlikely source of Chocoan plant lineages.
Hypotheses on the Mode and Tempo of Evolution of the Chocoan Flora
One peculiarity of the floristic composition of the Chocó is its high endemism that appears restricted to species, as there is only one endemic genus in the region (the recently described Sabinaria in the Arecaceae; Bernal and Galeano, 2013). To date, no endemic plant families have been found. Such curious patterns of endemism have attracted the attention of botanists for decades (Gentry, 1982b; Forero and Gentry, 1989; Galeano et al., 1998). Gentry’s seminal floristic work (1986) and the geological evidence available already nearly 50 years ago (Haffer, 1970), led him (Gentry, 1982a) to conclude that: a) extremely high levels of rainfall in the Chocó are linked to its rich plant diversity (Jaramillo, 2006) and its maintenance (Behling et al., 1998): b) Chocoan plant lineages may have derived from the Northern Andean region with possible contributions from Central America; c) the geological origin of the Chocó dates back to the middle Pleistocene and was part of the mountain building processes of the Colombian Andes. Thus, the floristic assembly of the Chocó, which benefitted from the biotic exchange between the actively rising Northern Andes and Central America, was supposedly formed Chocó from around 1 Mya onwards.
To test the scenarios postulated by Gentry, phylogenetic comparative methods based on densely sampled and representative phylogenies could reveal the age, direction and frequency of biotic exchanges between the Chocó and other regions (Pennington et al., 2004). In addition, they could inform on the role of geographical events that have shaped the diversification of the Chocoan flora (Winterton et al., 2014). A similar approach has been used to investigate the evolution of the savanna-adapted lineages in the South American Cerrado, which for plants is also very rich in species but is similarly depauperate at higher taxonomic levels of genera and families (Simon et al., 2009).
Few robust phylogenies for Chocoan plant species exist, and virtually all of them have sparse regional taxon sampling. Those available include Annonaceae [Cremastosperma (29 species/82% sampled - one Chocoan species) and Mosannona (14 species/78% sampled — one Chocoan species); Pirie et al., 2018], Bignoniaceae [Amphilophium (47 species/70% sampled — one Chocoan species); Thode et al., 2019], the two most species-rich groups of Neotropical orchids [tribe Cymbidieae (∼3,300 species/26% sampled — five Chocoan species) and subtribe Pleurothallidinae (∼5,100 species/11% sampled – four Chocoan species); Pérez-Escobar et al., 2017a] and Philodendron (Araceae, 560 species/30% sampled — 16 Chocoan species; Canal et al., 2019). These studies provide an excellent opportunity to test Gentry’s ideas of a Pleistocene Northern Andean–Central American origin for the Chocó flora as they include different levels of sampling (i.e. 10% to ∼80% of the known species diversity of each clade; the exact proportion of species diversity sampled is provided in Figure 2).
Figure 2 Temporal migration dynamics of selected Neotropical plant lineages represented in the Chocó and their diversification patterns. (A) Mean ages and their corresponding 95% High Density Probabilities (HDP) of Most Recent Common Ancestors (MRCA) of plant species/clades distributed in the Chocó. Ages estimates are colour coded by family and were obtained from published phylogenies of Cymbidieae and Pleurothallidinae (Orchidaceae: Pérez-Escobar et al., 2017a), Amphilophium (Bignoniaceae: Thode et al., 2019), Philodendron (Araceae: Canal et al., 2019), Cremastosperma and Mosannona (Annonaceae: Pirie et al., 2018). Boxplots representing mean values and quartiles were computed whenever four or more observations per plant family were available. [Inset: The proportion of species sampled vs the total known of species diversity in each surveyed phylogeny is provided with a picture of the corresponding plant family (Orchidaceae: Pleurothallis pierryi; Bignoniaceae: Anemopaegma orbiculatum; Araceae: Philodendron sp.; Annonaceae: Cremastosperma sp.)]. The green bar represents the approximate age of the terrestrial ecosystem onset in the Chocó. (B) A schematic phylogeny representing the two most prominent diversification patterns of Chocoan lineages, all of which appear to have occurred during the last ∼8 Ma: 1) migration from adjacent or distant biogeographical areas into the Chocó followed by in-situ diversification (blue arrows); 2) migration into the Chocó without subsequent diversification. Photos: O. Pérez.
Ancestral area estimations in Cymbidieae and Pleurothallidinae (Figures S1A, B–S2A and B) revealed eleven migrations to the Chocó, which could be classified into two patterns: a) Northern Andes to Chocó and b) Central America to Chocó. The first migration pattern included four dispersals from the late Miocene (∼6 Mya) to as recently as 2 Mya (Figures S1B and S2B). The second pattern consisted of four migrations from the adjacent Central America region towards the Chocó. Notably, most of these migrations occurred recently (∼2 Mya) and no recolonizations from the Chocó back to the Northern Andes or Central America were detected (Figures S1B and S2B). Ancestral character estimations of altitude as a continuous character indicated that most of the Northern Andean migrants were originally adapted to montane elevations, between elevations of ∼1,200 to 1,700 m. In contrast, most Central American migrants were probably first adapted to lowland environments. Descendent branches from Northern Andean migrants subsequently adapted to pre-montane environments (i.e. elevations below ∼500 m; Figures S1C and S2C). The putative montane-to-lowland adaptations into the Chocoan premontane environments revealed by our ACE analyses are striking, reflecting results reported for Chloranthaceae (Hedyosmum scaberrimum) and Magnoliaceae (e.g. Magnolia calimaensis) in the Chocó (Gentry, 1986; Galeano et al., 1998; Antonelli and Sanmartín, 2011b). Nevertheless, understanding how frequent the out-of-the-mountains migration pattern has been across Chocoan orchid lineages deserves further investigation.
Species level phylogenies of Cremastosperma, Mosannona, and Philodendron revealed similar modes and tempos of migration into the Chocó (Figure 2). Lineage colonizations occurred mostly after divergences from Central American and Andean ancestors between ∼7 and 1 Ma. Less frequent, alternative migration routes were recovered in Amphilophium and in the predominantly lowland epiphytic orchid Cycnoches (Pérez-Escobar et al., 2017b; Pérez-Escobar et al., 2017c). These are lineages from far-away biogeographical regions such as Amazonia which are, or have been, isolated from the Chocó by major geographical barriers (e.g. the Northern Andes). Here, a single migration from Amazonia towards the Chocó took place ∼1 Mya in Cycnoches (Pérez-Escobar et al., 2017c), whilst an older colonization (∼3 Ma) also from Amazonia occurred in Amphilophium (Thode et al., 2019).
The most common pattern of diversification across all surveyed lineages is that of colonization of the Chocó without subsequent in-situ speciation. Of the studies considered here, only two cases of migration followed by in-situ diversification were recovered, one in Anthurium (∼ 5 Mya, three species; Canal et al., 2019) and one in Cycnoches (∼1 Mya, two species; Pérez-Escobar, 2016). This apparent lack of in-situ plant diversification in the region might be explained by the very recent Pleistocene setting of the Chocoan modern landscape or alternatively, by the incomplete sampling of these phylogenies. However, the stark contrast between the high levels of species endemism and very limited number of genera restricted to the Chocó reflects the limited morphological divergence among lineages diversifying in the Chocó. Migrations without subsequent diversification is a remarkable pattern that is unusual in biodiversity hotspots [e.g. Tropical Andes, Seasonally Dry Forests and Indo-Pacific coral triangle (Pennington et al., 2009; DRYFLOR, 2016; Huang et al., 2017; Pérez-Escobar et al., 2018; Testo et al., 2019; Vasconcelos et al., 2019)] and deserves further study, for instance to investigate the possibility of cryptic species and on-going speciation. Additional phylogenies with representative sampling at species and population level in groups with disparate life histories and diversification rates could reveal how frequent in-situ diversification is and it is restricted to rapidly diversifying groups or not.
A Compound Origin of the Chocoan Flora
Phylogenetic comparative analyses of early Miocene lineages support Gentry’s idea of a Northern Andean–Central American origin of the Chocoan flora (Pérez-Escobar et al., 2017a). The proximity of the Northern Andean and Central American biogeographical regions to the Chocó could explain the mixed origin of the Chocó flora. However, phylogenetic frameworks of plant clades with a Pliocene age suggest that contributions from more distant biogeographical areas are also relevant for the assembly of the Chocó flora (Pérez-Escobar et al., 2017c). More recently, an example of a palm species with a cross-Chocoan distribution range and constant gene flow (Escobar et al., 2018) also support this migration pattern, indicating that the Chocó is a permeable, accessible region, not totally isolated from either adjacent or more distant biogeographical areas.
Late Miocene and Pliocene migrations into the Chocó might at first seem unlikely given that the modern lowland landscape of the Chocó region is not older than ∼3 Ma. However, the foothills of the Baudo in west Chocó and western Cordillera (west of the Uramita suture), also considered to be part of the Chocó region, are terrestrial landscapes established already by 6 Ma. As such, they could have served as a host area for plant migrants since the late Miocene. Thus, the timing of migrations into the Chocó, irrespective of the source biogeographical area, provides strong support for a predominantly Pleistocene geological onset of the region in South America, hence largely supporting Gentry’s assumption of a Pleistocene origin of the Chocó.
Taken together, these spatio-temporal dynamics suggest that a) allopatric speciation in plants promoted by the Andean uplift seems unlikely to have played a role in the formation of the Chocoan flora given the very recent age of migrations from biogeographical regions isolated by the Northern Andes and the older age (∼10 Ma) of formation of montane–alpine environments in the Northern part of the Cordillera (Hoorn et al., 2010; Hoorn and Wesselingh, 2010); b) Certain elements of Chocoan plant diversity have an earlier origin than Gentry thought, as evidenced by the early Pliocene migrations from the Northern Andes to the Chocó accounted in Pleurothallidinae; c) there has been strong biotic exchange between Central America and Chocó, particularly evident during the Pleistocene and seemingly unlinked with the timing of the closure of the Central American Seaway.
The current proximity of Central America to Chocó and/or the ability of orchid seeds to remain airborne for prolonged periods (Arditti and Ghani, 2000) and thus cover huge distances (Murren and Ellison, 1998) could explain the high frequency of biotic exchange between Central America and the Chocó for this family. The timing of the onset of modern Chocoan landscapes in turn could account for the similar age of these migrations (ranging from ∼5–2 Mya). Moreover, biotic contributions from other biogeographical areas like Amazonia also took place, suggesting that the Chocoan flora could be derived as well, but to a lesser extent, from other regions than just the Northern Andes and Central America.
Geological History and Floristic Similarities of Central America and the Chocó Block
The floristic affinities of the Chocó and Central America have puzzled botanists for decades. Gentry’s notion of Central American contributions to the origin of the Chocoan flora was based on his observations of the striking resemblance of the species diversity shared between both regions in key family groups, including Annonaceae and Rubiaceae (Gentry, 1982b; Gentry, 1986). The composition of the orchid diversity in Central America mirrors in part that of the Chocó. Particularly, groups from the subtribe Zygopetalinae (e.g. Houlletia, Huntleya) and Maxillariinae (e.g. Camaridium) that peak in species-richness in the lowland and premontane regions of Central America, are also well represented in the Chocó (Urreta, 2005; Bogarín et al., 2015; Kirby, 2016). Comparative floristic studies focused on selected Araceae genera and pteridophytes in general (Lellinger, 1975) are in line with these findings (Mora et al., 2006), and an older idea of a more expansive Chocó region that extended to the Nicaraguan–Costa Rican border has received some support (Lellinger and de la Sota, 1978). However, distribution patterns of palms revealed that the Darien Gap on the Panama–Colombian border is an effective dispersal barrier for bidirectional exchange between South and Central America, at least for lineages that are dependent on other organisms for dispersal (Cano et al., 2017).
The Central American origin of one part of the Chocó region might provide a more plausible explanation for the strong floristic affinity between Central America and the Chocó. Here, the few chronograms of plant lineages available provide evidence of floristic admixture between Central and South America triggered by the accretion of the Chocó block. Comparable admixture has been suggested for other merging landmasses, such as the Indian subcontinent colliding with Asia (Thornhill et al., 2015). Intermingled clades of Dussia in the Leguminosae distributed in Central America and the Chocó suggest a nearly simultaneous migration from Central America into the Chocó, with concomitant diversification towards the late Miocene (Winterton et al., 2014). A similar late Pliocene migration pattern has been reported in Cremastosperma (Annonaceae), where a Central American common ancestor appears to have given rise to an endemic Central American and Chocoan species pair (C. westrae and C. novogranatense, respectively; Pirie et al., 2018). However, whether similar diversification patterns occur in other prominent plant lineages with more localized modes of dispersal remains to be tested.
New Research Questions and Future Directions
Our detailed evaluation of migrations-through-time of selected Neotropical plant lineages between Neotropical regions and the Chocó reveals that the assembly of the Chocó flora is more complex than first proposed by Gentry. Whilst the timing of the onset of modern Chocoan landscapes could explain the poor endemism of genera and families in the Chocó, the influence of other biotic and abiotic variables in the diversification of the Chocoan flora remains unclear and begs further investigation. These include evaluating a potential role of extinction (within and between clades, and in relation to landscape changes) in shaping the endemicity of the Chocó region and understanding the influence of historical changes in humidity and precipitation on speciation. Moreover, future studies should focus on understanding the diversity and affinity of poorly studied but prominent plant lineages of the Central American floras, to facilitate comparison of floristic affinities and biotic interchange. Detailed floristic studies in the northern and southern fringes of the Chocó could further clarify the delimitation of the Chocó biogeographic region by comparing similarities in distribution patterns across plant groups (Vilhena and Antonelli, 2015).
Solid phylogenomic frameworks coupled with reliable distribution data of plant groups well represented in Central America and the Chocó (such as Araceae, Fabaceae, Myrtaceae, Melastomataceae, Moraceae, Rubiaceae, and Urticaceae) should help tease apart the role of modes of dispersal, plant habit, or life-form in determining the mode and tempo of plant migrations into the Chocó. For example, biogeographical studies of densely species level phylogenies could distinguish the roles of montane areas in the Northern Andean cordilleras and the Panamanian magmatic arcs (i.e. the western Cordillera in South America) as source of migrants into the Chocó lowlands. Furthermore, it would be particularly interesting to investigate in more detail the impact of the accretion of the Chocó Block to the South American plate. That event must not only have facilitated plant dispersal (Bacon et al., 2015) but also led to important climatic and edaphic changes in the Chocó. The direction and frequency of migrations across multiple plant lineages, coupled with changes in diversification rates and their relation to environmental changes, would shed further light onto the assembly of the Chocoan flora and its dynamics.
Author Contributions
OP-E, SM, EL, AM, and AA designed the research. OP-E, CJ, EL, SM, AA, DB, DG, SD, JA-C, ASM, and AA performed the research. OP-E, CJ, EL, AM, and AA wrote the paper with contributions from all authors.
Funding
OP-E is supported by the Lady Sainsbury Orchid Fellowship at the Royal Botanic Gardens, Kew.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
We are grateful to Rodrigo Cámara-Leret for sharing pictures of the Chocó rain forest and Alexandre Zuntini for identifying the Anemopaegma species shown in Figure 2. Two reviewers and the associate editor provided valuable feedback that enhanced an earlier version of this manuscript.
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2019.01328/full#supplementary-material
Figure S1 | Spatio-temporal macro-evolutionary dynamics of the orchid clade Pleurothallidinae in the American tropics (adapted from Pérez-Escobar et al., 2017a). (A) Bayesian chronogram of the Pleurothallidinae, with ancestral areas estimated at nodes using the Dispersal, Extinction and Cladogenesis and the founder speciation event models as implemented in the R package BioGeoBEARS. Branches are colour coded according to the ancestral area estimated at the parent node. The distribution of the species sampled in the phylogeny is shown as horizontal colour coded bars in front of the tree terminals. Four clades with nested migration events to the Chocó region are highlighted in grey and labelled with roman numerals (I–IV). (B). Zoom-in on clades with nested migrations to the Chocó region (highlighted with pink arrows) (Inset: a: Anathallis funerea, b: Anathallis lewisiae, c: Dracula sp., d: Specklinia pereziana, e: Specklinia berolinensis). Letters at nodes represent inferred ancestral areas. (C) Maximum Likelihood Ancestral State Estimation analyses of altitude as a continuous variable of clades I-IV using a Brownian motion model as implemented in the R package phytools. Branches are colour coded as a function of estimated ancestral altitude values. [Inset: mean paleo-elevation of Central, Northern and Venezuelan Andes during the last 11 Ma (modified from Hoorn et al., 2010); biogeographical regions employed for ancestral area estimation analyses. The delimitation of the Chocó follows that of (Antonelli et al., 2009)]. Photos: D. Bogarín, A. Karremans, O. Pérez-Escobar.
Figure S2 | Spatio-temporal macro-evolutionary dynamics of the orchid clade Cymbidieae in the American tropics (adapted from Pérez-Escobar et al., 2017a). (A) Bayesian chronogram of the Pleurothallidinae, with ancestral areas estimated at nodes using the Dispersal, Extinction and Cladogenesis and the founder speciation event models as implemented in the R package BioGeoBEARS. Branches are colour coded according to the ancestral area estimated at the parent node. The distribution of the species sampled in the phylogeny is shown as horizontal colour coded bars in front of the tree terminals. Three clades with nested migration events to the Chocó region are highlighted in grey and labelled with roman numerals (I–III). (B). Zoom-in of clades with nested migrations to the Chocó region (highlighted with pink arrows) (Inset: a: Cycnoches lehmannii, b: Dichaea globosa, c: Euryblema andreae, d: Chaubardiella pubescens, e: Gongora sp.). Letters at nodes represent inferred ancestral areas. (C) Maximum Likelihood Ancestral State Estimation analyses of altitude as a continuous variable of clades I-III using a Brownian motion model as implemented in the R package phytools. Branches are colour coded as a function of estimated ancestral altitude values. [Inset: mean paleo-elevation of Central, Northern and Venezuelan Andes during the last 11 Ma (modified from Hoorn et al., 2010); biogeographical regions employed for ancestral area estimation analyses. The delimitation of the Chocó follows that of (Antonelli et al., 2009)]. Photos: D. Bogarín, O. Pérez-Escobar.
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Keywords: biogeography, macroevolution, neotropical region, hyper-diversity, Andean uplift, Chocó, Central America
Citation: Pérez-Escobar OA, Lucas E, Jaramillo C, Monro A, Morris SK, Bogarín D, Greer D, Dodsworth S, Aguilar-Cano J, Sanchez Meseguer A and Antonelli A (2019) The Origin and Diversification of the Hyperdiverse Flora in the Chocó Biogeographic Region. Front. Plant Sci. 10:1328. doi: 10.3389/fpls.2019.01328
Received: 27 April 2019; Accepted: 24 September 2019;
Published: 06 December 2019.
Edited by:
Thomas L.P. Couvreur, IRD UMR232 Diversité, adaptation, développement des plantes (DIADE), FranceReviewed by:
Weston Testo, University of Vermont, United StatesColin Hughes, University of Zurich, Switzerland
Copyright © 2019 Pérez-Escobar, Lucas, Jaramillo, Monro, Morris, Bogarín, Greer, Dodsworth, Aguilar-Cano, Sanchez Meseguer and Antonelli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Oscar Alejandro Pérez-Escobar, o.perez-escobar@kew.org
†These authors have contributed equally to this work