AUTHOR=Furuya Tomoyuki , Kimori Yoshitaka , Tsukaya Hirokazu TITLE=A Method for Evaluating Three-Dimensional Morphological Features: A Case Study Using Marchantia polymorpha JOURNAL=Frontiers in Plant Science VOLUME=10 YEAR=2019 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01214 DOI=10.3389/fpls.2019.01214 ISSN=1664-462X ABSTRACT=
The description and evaluation of morphological features are essential to many biological studies. Bioimaging and quantification methods have been developed to analyze the morphological features of plants. However, efficient three-dimensional (3D) imaging and its quantification are still under development, particularly for studies of plant morphology, due to complex organ structure with great flexibility among individuals with the same genotype. In this study, we propose a new approach that combines a 3D imaging technique using micro-computed tomography and a mathematical image-processing method to describe 3D morphological features. As an example, we applied this method to