AUTHOR=Liu Siwen , Wu Bo , Lv Shuxia , Shen Zongzhuan , Li Rong , Yi Ganjun , Li Chunyu , Guo Xiuwu 

TITLE=Genetic Diversity in FUB Genes of Fusarium oxysporum f. sp. cubense Suggests Horizontal Gene Transfer

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01069

DOI=10.3389/fpls.2019.01069

ISSN=1664-462X

ABSTRACT=<p>Fusaric acid (FA) is an important secondary metabolite of many Fusarium species and involved in the wilt symptoms caused in banana by <italic>Fusarium oxysporum</italic> f. sp. <italic>cubense (Foc)</italic>. To investigate the evolution characteristics of the 12 <italic>Foc</italic> FA biosynthetic genes (<italic>FUB</italic>), coding sequences of the 12 <italic>FUB</italic> genes and three housekeeping genes, <italic>EF-1</italic>α<italic>/RPB1/RPB2</italic> (translation elongation factor-1α/RNA polymerase II subunit I/RNA polymerase II subunit II), were subjected to genetic diversity analysis, phylogenetic analysis, recombination detection, and selective pressure analysis. The results of selective pressure analysis showed that the 15 genes were mainly subjected to negative selection. However, a significantly higher number of silent mutations, which could not be simply explained by selective pressure difference, were observed in the 12 <italic>FUB</italic> genes in <italic>Foc</italic> than in the three housekeeping genes. Infraspecies phylogeny and recombination detection analysis showed that significantly more horizontal gene transfer (HGT) events (normalized) had occurred in the <italic>FUB</italic> genes than in the three housekeeping genes. In addition, many of these events involved outgroup isolates and significantly increased the genetic diversity of <italic>FUB</italic> genes in <italic>Foc</italic>. The infraspecies phylogenetic analysis suggested that the polyphyletic phylogeny proposed for Foc requires further discussion, and the divergence of race 1, race 4, and the common ancestor of several <italic>F. oxysporum</italic> (<italic>Fo</italic>) isolates pathogenic to nonbanana plants should have diverged over a short period. Finally, our results suggest that the <italic>FUB</italic> genes in <italic>Fo</italic> should have benefited from HGT to gain a relatively high genetic diversity to respond to different host plants and environments despite mainly being subject to negative selection.</p>