AUTHOR=Araujo Ricardo , Dunlap Christopher , Barnett Steve , Franco Christopher M.M. 

TITLE=Decoding Wheat Endosphere–Rhizosphere Microbiomes in Rhizoctonia solani–Infested Soils Challenged by Streptomyces Biocontrol Agents

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01038

DOI=10.3389/fpls.2019.01038

ISSN=1664-462X

ABSTRACT=<p>The endosphere and the rhizosphere are pertinent milieus with microbial communities that perturb the agronomic traits of crop plants through beneficial or detrimental interactions. In this study, we challenged these communities by adding <italic>Streptomyces</italic> biocontrol strains to wheat seeds in soils with severe <italic>Rhizoctonia solani</italic> infestation. Wheat plants were grown in a glasshouse standardized system, and the bacterial and fungal microbiomes of 233 samples of wheat roots (endosphere) and rhizosphere soils were monitored for 20 weeks, from seed to mature plant stage. The results showed highly dynamic and diverse microbial communities that changed over time, with <italic>Sphingomonas</italic> bacteria and <italic>Aspergillus</italic>, <italic>Dipodascus</italic>, and <italic>Trichoderma</italic> fungi increasing over time. Application of biocontrol <italic>Streptomyces</italic> strains promoted plant growth and maturation of wheat heads and modulated the root microbiome, decreasing <italic>Paenibacillus</italic> and increasing other bacterial and fungal OTUs. The soils with the highest levels of <italic>R. solani</italic> had increased reads of <italic>Thanatephorus</italic> (<italic>Rhizoctonia</italic> anamorph) and increased root disease levels and increased <italic>Balneimonas</italic>, <italic>Massilia</italic>, <italic>Pseudomonas</italic>, and unclassified <italic>Micrococcaceae</italic>. As we enter the era of biologically sustainable agriculture, it may be possible to reduce and limit the effects of serious fungal infestations by promoting a beneficial microbiome through the application of biocontrol agents during different periods of plant development.</p>