AUTHOR=Zhao Meng-Jie , Yin Li-Juan , Ma Jian , Zheng Jia-Cheng , Wang Yan-Xia , Lan Jin-Hao , Fu Jin-Dong , Chen Ming , Xu Zhao-Shi , Ma You-Zhi 

TITLE=The Roles of GmERF135 in Improving Salt Tolerance and Decreasing ABA Sensitivity in Soybean

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.00940

DOI=10.3389/fpls.2019.00940

ISSN=1664-462X

ABSTRACT=<p>Abscisic acid (ABA) mediates various abiotic stress responses, and ethylene responsive factors (ERFs) play vital role in resisting stresses, but the interaction of these molecular mechanisms remains elusive. In this study, we identified an ABA-induced soybean ERF gene <italic>GmERF135</italic> that was highly up-regulated by ethylene (ET), drought, salt, and low temperature treatments. Subcellular localization assay showed that the GmERF135 protein was targeted to the nucleus. Promoter <italic>cis</italic>-acting elements analysis suggested that numerous potential stress responsive <italic>cis</italic>-elements were distributed in the promoter region of <italic>GmERF135</italic>, including ABA-, light-, ET-, gibberellin (GA)-, and methyl jasmonate (MeJA)-responsive elements. Overexpression of <italic>GmERF135</italic> in <italic>Arabidopsis</italic> enhanced tolerance to drought and salt conditions. In addition, <italic>GmERF135</italic> promoted the growth of transgenic hairy roots under salt and exogenous ABA conditions. These results suggest that soybean GmERF135 may participate in both ABA and ET signaling pathways to regulate the responses to multiple stresses.</p>