AUTHOR=Thode Verônica A. , Lohmann Lúcia G. 

TITLE=Comparative Chloroplast Genomics at Low Taxonomic Levels: A Case Study Using Amphilophium (Bignonieae, Bignoniaceae)

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.00796

DOI=10.3389/fpls.2019.00796

ISSN=1664-462X

ABSTRACT=<p>Chloroplast (cp) genome organization, gene order, and content have long been considered conserved among land plants. Despite that, the generation of thousands of complete plastomes through next-generation sequencing (NGS) has challenged their conserved nature. In this study, we analyze 11 new complete plastomes of <italic>Amphilophium</italic> (Bignonieae, Bignoniaceae), a diverse genus of Neotropical lianas, and that of <italic>Anemopaegma prostratum</italic>. We explored the structure and content of the assembled plastomes and performed comparative analyses within <italic>Amphilophium</italic> and among other plastomes available for Bignoniaceae. The overall gene content and orientation of plastomes is similar in all species studied. Plastomes are not conserved among <italic>Amphilophium</italic>, showing significant differences in length (155,262–164,786 bp), number of genes duplicated in the IRs (eight, 18, or 19), and location of the SC/IR boundaries (i.e., LSC/IRa junction between <italic>rps19</italic> and <italic>rpl2</italic> genes, within <italic>petD</italic>, or within <italic>petB</italic>). Length differences reflect expansions of the IRs and contractions of the LSC regions. The plastome of <italic>A. prostratum</italic> is 168,172 bp, includes 19 duplicated genes, and has the LSC/IRa boundary located within the <italic>petB</italic> gene. <italic>Amphilophium</italic> plastomes show high nucleotide diversity, with many hypervariable regions, and 16 genes with signatures of positive selection. Multiple SSRs and repeat regions were identified for <italic>Amphilophium</italic> and <italic>Anemopaegma prostratum</italic>. The differences in structure detected within <italic>Amphilophium</italic> plastomes in terms of LSC/IR and IR/SSC boundaries, number of duplicated genes, and genome sizes are mostly shared between taxa that belong to the same clade. Our results bring new insights into the evolution of plastomes at low taxonomic levels.</p>