AUTHOR=Gouhier-Darimont Caroline , Stahl Elia , Glauser Gaetan , Reymond Philippe 

TITLE=The Arabidopsis Lectin Receptor Kinase LecRK-I.8 Is Involved in Insect Egg Perception

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.00623

DOI=10.3389/fpls.2019.00623

ISSN=1664-462X

ABSTRACT=<p>Plants induce defense responses after insect egg deposition, but very little is known about the perception mechanisms. In <italic>Arabidopsis thaliana</italic>, eggs of the specialist insect <italic>Pieris brassicae</italic> trigger accumulation of reactive oxygen species (ROS) and salicylic acid (SA), followed by induction of defense genes and localized necrosis. Here, the involvement of the clade I L-type lectin receptor kinase LecRK-I.8 in these responses was studied. Expression of <italic>LecRK-I.8</italic> was upregulated at the site of <italic>P. brassicae</italic> oviposition and egg extract (EE) treatment. ROS, SA, cell death, and expression of <italic>PR1</italic> were substantially reduced in the <italic>Arabidopsis</italic> knock-out mutant <italic>lecrk-I.8</italic> after EE treatment. In addition, EE-induced systemic resistance against <italic>Pseudomonas syringae</italic> was abolished in <italic>lecrk-I.8</italic>. Expression of ten clade I homologs of <italic>LecRK-I.8</italic> was also induced by EE treatment, but single mutants displayed only weak alteration of EE-induced <italic>PR1</italic> expression. These results demonstrate that LecRK-I.8 is an early component of egg perception.</p>