AUTHOR=Wu Jiandong , Chen Long , Chen Mingchao , Zhou Wei , Dong Qing , Jiang Haiyang , Cheng Beijiu 

TITLE=The DOF-Domain Transcription Factor ZmDOF36 Positively Regulates Starch Synthesis in Transgenic Maize

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.00465

DOI=10.3389/fpls.2019.00465

ISSN=1664-462X

ABSTRACT=<p>Starch synthesis is a complex process that influences crop yield and grain quality in maize. Many key enzymes have been identified in starch biosynthesis; however, the regulatory mechanisms have not been fully elucidated. In this study, we identified a DOF family gene, <italic>ZmDOF36</italic>, through transcriptome sequencing analysis. Real-time PCR indicated that <italic>ZmDOF36</italic> was highly expressed in maize endosperm, with lower expression in leaves and tassels. <italic>ZmDOF36</italic> is a typical DOF transcription factor (TF) that is localized to the nucleus and possesses transcriptional activation activity, and its transactivation domain is located in the C-terminus (amino acids 227–351). Overexpression of <italic>ZmDOF36</italic> can increase starch content and decrease the contents of soluble sugars and reducing sugars. In addition, abnormal starch structure in transgenic maize was also observed by scanning electron microscopy (SEM). Furthermore, the expression levels of starch synthesis-related genes were up-regulated in <italic>ZmDOF36</italic>-expressing transgenic maize. ZmDOF36 was also shown to bind directly to the promoters of six starch biosynthesis genes, <italic>ZmAGPS1a</italic>, <italic>ZmAGPL1</italic>, <italic>ZmGBSSI</italic>, <italic>ZmSSIIa</italic>, <italic>ZmISA1</italic>, and <italic>ZmISA3</italic> in yeast one-hybrid assays. Transient expression assays showed that ZmDOF36 can activate the expression of <italic>ZmGBSSI</italic> and <italic>ZmISA1</italic> in tobacco leaves. Collectively, the results presented here suggest that ZmDOF36 acts as an important regulatory factor in starch synthesis, and could be helpful in devising strategies for modulating starch production in maize endosperm.</p>