AUTHOR=Liu Siyu , Zheng Lu , Jia Jia , Guo Jia , Zheng Mengdi , Zhao Jun , Shao Jingxia , Liu Xiayan , An Lijun , Yu Fei , Qi Yafei 

TITLE=Chloroplast Translation Elongation Factor EF-Tu/SVR11 Is Involved in var2-Mediated Leaf Variegation and Leaf Development in Arabidopsis

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.00295

DOI=10.3389/fpls.2019.00295

ISSN=1664-462X

ABSTRACT=<p>Chloroplasts are semiautonomous organelles, retaining their own genomes and gene expression apparatuses but controlled by nucleus genome encoded protein factors during evolution. To analyze the genetic regulatory network of FtsH-mediated chloroplast development in <italic>Arabidopsis</italic>, a set of suppressor mutants of <italic>yellow variegated</italic> (<italic>var2</italic>) have been identified. In this research, we reported the identification of another new <italic>var2</italic> suppressor locus, <italic>SUPPRESSOR OF VARIEGATION11</italic> (<italic>SVR11</italic>), which encodes a putative chloroplast-localized prokaryotic type translation elongation factor EF-Tu. <italic>SVR11</italic> is likely essential to chloroplast development and plant survival. <italic>GUS</italic> activity reveals that <italic>SVR11</italic> is abundant in the juvenile leaf tissue, lateral roots, and root tips. Interestingly, we found that <italic>SVR11</italic> and <italic>SVR9</italic> together regulate leaf development, including leaf margin development and cotyledon venation patterns. These findings reinforce the notion that chloroplast translation state triggers retrograde signals regulate not only chloroplast development but also leaf development.</p>