AUTHOR=Bett Bosibori , Gollasch Stephanie , Moore Andy , Harding Robert , Higgins Thomas J. V. 

TITLE=An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.00219

DOI=10.3389/fpls.2019.00219

ISSN=1664-462X

ABSTRACT=<p>An improved cowpea transformation method utilizing <italic>Agrobacterium</italic>-mediated gene delivery to explants derived from the cotyledonary nodes of imbibed cowpea seed is described. The explants were regenerated following a sonication procedure and a stringent selection comprising alternating regimes of kanamycin and geneticin. The method was reproducible and led to the recovery of independent fertile transgenic plants in the greenhouse at a level of about one per cent of starting explants. A transgene encoding an insecticidal protein from <italic>Bacillus thuringiensis</italic> was used to demonstrate the efficacy of the system.</p>