AUTHOR=Sharma Anket , Kumar Vinod , Yuan Huwei , Kanwar Mukesh Kumar , Bhardwaj Renu , Thukral Ashwani Kumar , Zheng Bingsong 

TITLE=Jasmonic Acid Seed Treatment Stimulates Insecticide Detoxification in Brassica juncea L.

JOURNAL=Frontiers in Plant Science

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2018.01609

DOI=10.3389/fpls.2018.01609

ISSN=1664-462X

ABSTRACT=<p>The present study focused on assessing the effects of jasmonic acid (JA) seed treatment on the physiology of <italic>Brassica juncea</italic> seedlings grown under imidacloprid (IMI) toxicity. It has been observed that IMI application declined the chlorophyll content and growth of seedlings. However, JA seed treatment resulted in the significant recovery of chlorophyll content and seedling growth. Contents of oxidative stress markers like superoxide anion, hydrogen peroxide, and malondialdehyde were enhanced with IMI application, but JA seed treatment significantly reduced their contents. Antioxidative defense system was activated with IMI application which was further triggered after JA seed treatment. Activities of antioxidative enzymes and contents of non-enzymatic antioxidants were enhanced with the application of IMI as well as JA seed treatment. JA seed treatment also regulated the gene expression of various enzymes under IMI stress. These enzymes included respiratory burst oxidase (<italic>RBO</italic>), Ribulose-1,5-bisphosphate carboxylase/oxygenase (<italic>RUBISCO</italic>), NADH-ubiquinone oxidoreductase (<italic>NADH</italic>), carboxylesterase (<italic>CXE</italic>), chlorophyllase (<italic>CHLASE</italic>), cytochrome P450 monooxygenase (<italic>P450</italic>). JA seed treatment up-regulated the expressions of <italic>RUBISCO, NADH, CXE</italic>, and <italic>P450</italic> under IMI toxicity. However, expressions of <italic>RBO</italic> and <italic>CHLASE</italic> were down-regulated in seedlings germinated from JA seed treatment and grown in presence of IMI. Seed soaking with JA also resulted in a significant reduction of IMI residues in <italic>B. juncea</italic> seedlings. The present study concluded that seed soaking with JA could efficiently reduce the IMI toxicity by triggering the IMI detoxification system in intact plants.</p>