AUTHOR=Izhaki Anat , Alvarez John P. , Cinnamon Yuval , Genin Olga , Liberman-Aloni Raya , Eyal Yoram 

TITLE=The Tomato BLADE ON PETIOLE and TERMINATING FLOWER Regulate Leaf Axil Patterning Along the Proximal-Distal Axes

JOURNAL=Frontiers in Plant Science

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2018.01126

DOI=10.3389/fpls.2018.01126

ISSN=1664-462X

ABSTRACT=<p>Leaf axil patterning occurs concomitantly with leaf development and takes place at the boundary zone which demarcates the initiating leaf primordium from the shoot apical meristem. Subsequent growth and differentiation result in establishment of the axillary meristem and abscission zone (AZ) along the proximal-distal axis of the leaf axil, yet the molecular mechanisms that regulate these events are poorly understood. We studied the role of the tomato <italic>BLADE ON PETIOLE</italic> (<italic>SlBOP</italic>) boundary gene family on the development of the leaf axil using <italic>BOP</italic>-silenced plants as well as <italic>BOP</italic>-mutated lines. We show that silencing of the tomato <italic>SlBOP</italic> gene family affects patterning of the leaf axil along the proximal-distal axis, manifested by dispositioning of the AM and abnormal development of the adjacent tissue resulting in lack of a functional leaf AZ. Dissection of the role of each of the three tomato <italic>SlBOPs</italic> by analysis of single, double and triple null-mutants demonstrated that <italic>SlBOP2</italic> is the dominant gene in leaf axil patterning, but does not rule out involvement of <italic>SlBOP1</italic> and <italic>SlBOP3</italic> in correct AM positioning. We further studied the potential role of TERMINATING FLOWER (TMF), a transcription factor which was previously shown to interact with SlBOPs, in leaf axil patterning using TMF mutant tomato lines. The results suggest that similar to SlBOP2, TMF is involved in leaf axil proximal-distal patterning and AZ development.</p>