AUTHOR=Zhang Tengxun , Sun Ming , Guo Yanhong , Shi Xuejun , Yang Yongjuan , Chen Juntong , Zheng Tangchun , Han Yu , Bao Fei , Ahmad Sagheer 

TITLE=Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers

JOURNAL=Frontiers in Plant Science

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2018.00909

DOI=10.3389/fpls.2018.00909

ISSN=1664-462X

ABSTRACT=<p><italic>Lilium</italic>, the famous and significant cut flower, emits a variety of volatile organic compounds, which mainly contain monoterpenes, such as myrcene, (E)-β-ocimene, and linalool. To understand the molecular mechanism of monoterpene synthesis in <italic>Lilium</italic>, we cloned two potential genes in the methylerythritol 4-phosphate pathway, namely <italic>LiDXS</italic> and <italic>LiDXR</italic>, from the strong-flavored oriental <italic>Lilium</italic> ‘Siberia’ using a homology-based PCR strategy. The expression levels of <italic>LiDXS</italic> and <italic>LiDXR</italic> were consistent with the emission and accumulation of monoterpenes in different floral organs and during the floral development, indicating that these two genes may play key roles in monoterpene synthesis. Subcellular localization demonstrated that LiDXS and LiDXR are expressed in the chloroplasts. Ectopic expression in transgenic tobacco suggested that the flowers of <italic>LiDXS</italic> and <italic>LiDXR</italic> transgenic lines accumulated substantially more diterpene, sclareol, compared to the plants transformed with empty vector. Surprisingly, increased content of the monoterpene, linalool and sesquiterpene, caryophyllene, were detected in the <italic>LiDXR</italic> transgenic lines, whereas the emission of caryophyllene, increased in one of the <italic>LiDXS</italic> transgenic tobacco lines, indicating that these two genes play significant roles in the synthesis of floral volatiles in the transgenic plants. These results demonstrate that <italic>LiDXR</italic> can contribute to monoterpene biosynthesis in <italic>Lilium</italic> ‘Siberia’; however, the role of <italic>LiDXS</italic> in the biosynthesis of monoterpenes needs further study.</p>