AUTHOR=Zhu Bao-Hua , Zhang Rui-Hao , Lv Na-Na , Yang Guan-Pin , Wang Yi-Sheng , Pan Ke-Hou 

TITLE=The Role of Malic Enzyme on Promoting Total Lipid and Fatty Acid Production in Phaeodactylum tricornutum

JOURNAL=Frontiers in Plant Science

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2018.00826

DOI=10.3389/fpls.2018.00826

ISSN=1664-462X

ABSTRACT=<p>To verify the function of malic enzyme (ME1), the ME1 gene was endogenously overexpressed in <italic>Phaeodactylum tricornutum</italic>. Overexpression of <italic>ME1</italic> increased neutral and total lipid content and significantly increased saturated fatty acids (SFAs) and polyunsaturated fatty acids (PUFAs) in transformants, which varied between 23.19 and 25.32% in SFAs and between 49.02 and 54.04% in PUFAs, respectively. Additionally, increased ME1 activity was accompanied by elevated NADPH content in all three transformants, indicating that increased ME1 activity produced additional NADPH comparing with that of WT. These results indicated that ME1 activity is NADP-dependent and plays an important role in the NADPH levels required for lipid synthesis and fatty acid desaturation in <italic>P. tricornutum</italic>. Furthermore, our findings suggested that overexpression of endogenous <italic>ME1</italic> represents a valid method for boosting neutral-lipid yield in diatom.</p>